dc.contributor.author |
Rana, Sadia Jafar |
|
dc.contributor.author |
Hussain Shah, Syed Nisar |
|
dc.contributor.author |
Mudassir, Jahanzeb |
|
dc.contributor.author |
Shahzad, Aqeel |
|
dc.date.accessioned |
2022-12-05T03:23:53Z |
|
dc.date.available |
2022-12-05T03:23:53Z |
|
dc.date.issued |
2019-11-02 |
|
dc.identifier.citation |
Rana, S. J., Shah, S. N. H., Mudassir, J., & Shahzad, A. (2019). RP-HPLC based method for the determination of Tamsulosin HCl in API, Prepared dosage form and spiked plasma. Pakistan Journal of Pharmaceutical Sciences, 32. |
en_US |
dc.identifier.issn |
1011-601X |
|
dc.identifier.uri |
http://142.54.178.187:9060/xmlui/handle/123456789/14587 |
|
dc.description.abstract |
An analytical method for measurement of tamsulosin HCl from its different life cycle stages was developed
using RP-HPLC technique. The elution of tamsulsoin was studied using MediterraneaTM Sea 18 column (dimensions 250×4.6 mm and pore size 5µm) and mobile phase comprising of buffer (pH 5.4): Acetonitrile (ACN) at ratio 60:40. The tamsolusin HCl elution was also studied by varying the operating conditions including the composition and flow rate of mobile phase, stationary phase temperature and scanning wavelength. The optimal elution conditions include; a) mobile phase flow rate; 1ml/min, b) wavelength; 224nm and stationary phase temperature 30°C. Linearity was recorded in the absorption data over tamsulosin concentration range of 0.007 to 40µg/ml. The values of paramters LOD and LOQ noted for tamsulosin dissolved in mobile phase were 0.0014 and 0.042µg/ml respectively, while for the counterpart in spiked plasma were 0.0017 and 0.051µg/ml respectively. The analytical method was prompt, accurate, reproducible and suitable for analysis of tamsulosin HCl in different samples of interest at formulation, regulation and clinical evaluations. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Karachi: Faculty of Pharmacy & Pharmaceutical Sciences, Karachi |
en_US |
dc.subject |
Tamsulosin HCl |
en_US |
dc.subject |
spiked plasma |
en_US |
dc.subject |
acetonitrile |
en_US |
dc.title |
RP-HPLC based method for the determination of Tamsulosin HCl in API, Prepared dosage form and spiked plasma |
en_US |
dc.type |
Article |
en_US |