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RP-HPLC based method for the determination of Tamsulosin HCl in API, Prepared dosage form and spiked plasma

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dc.contributor.author Rana, Sadia Jafar
dc.contributor.author Hussain Shah, Syed Nisar
dc.contributor.author Mudassir, Jahanzeb
dc.contributor.author Shahzad, Aqeel
dc.date.accessioned 2022-12-05T03:23:53Z
dc.date.available 2022-12-05T03:23:53Z
dc.date.issued 2019-11-02
dc.identifier.citation Rana, S. J., Shah, S. N. H., Mudassir, J., & Shahzad, A. (2019). RP-HPLC based method for the determination of Tamsulosin HCl in API, Prepared dosage form and spiked plasma. Pakistan Journal of Pharmaceutical Sciences, 32. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/14587
dc.description.abstract An analytical method for measurement of tamsulosin HCl from its different life cycle stages was developed using RP-HPLC technique. The elution of tamsulsoin was studied using MediterraneaTM Sea 18 column (dimensions 250×4.6 mm and pore size 5µm) and mobile phase comprising of buffer (pH 5.4): Acetonitrile (ACN) at ratio 60:40. The tamsolusin HCl elution was also studied by varying the operating conditions including the composition and flow rate of mobile phase, stationary phase temperature and scanning wavelength. The optimal elution conditions include; a) mobile phase flow rate; 1ml/min, b) wavelength; 224nm and stationary phase temperature 30°C. Linearity was recorded in the absorption data over tamsulosin concentration range of 0.007 to 40µg/ml. The values of paramters LOD and LOQ noted for tamsulosin dissolved in mobile phase were 0.0014 and 0.042µg/ml respectively, while for the counterpart in spiked plasma were 0.0017 and 0.051µg/ml respectively. The analytical method was prompt, accurate, reproducible and suitable for analysis of tamsulosin HCl in different samples of interest at formulation, regulation and clinical evaluations. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences, Karachi en_US
dc.subject Tamsulosin HCl en_US
dc.subject spiked plasma en_US
dc.subject acetonitrile en_US
dc.title RP-HPLC based method for the determination of Tamsulosin HCl in API, Prepared dosage form and spiked plasma en_US
dc.type Article en_US


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