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PRIMARY AND SECONDARY SOMATIC EMBRYOGENESIS FROM LEAF EXPLANTS OF ROSEMARY (ROSMARINUS OFFICINALIS L. - LAMIACEAE)

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dc.contributor.author NOURIN AMAN, NOURIN AMAN
dc.contributor.author HUMERA AFRASIAB, HUMERA AFRASIAB
dc.date.accessioned 2022-12-05T05:18:20Z
dc.date.available 2022-12-05T05:18:20Z
dc.date.issued 2014-06-19
dc.identifier.citation Aman, N., & Afrasiab, H. (2014). Primary and secondary somatic embryogenesis from leaf explants of rosemary (Rosmarinus officinalis L.-Lamiaceae). Pak J Bot, 46, 903-909. en_US
dc.identifier.issn 2070-3368
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/14617
dc.description.abstract Somatic embryogenesis was obtained from young leaf explants of rosemary with 2, 4-Dichlorophenoxyaceti acid (2, 4- D) in different concentrations (0.45, 2.25, 4.52, 6.75, 9.04 µM) in combination with Benzyladenine (BA) using modified woody plant medium (WPM). The best frequencies of induction were obtained in a medium supplemented with 2.25 µM 2, 4-D and 0.45 µM BA, in which 100% of the calli produced somatic embryos. Subculture of primary somatic embryos on modified WPM without hormones gave rise to clusters with secondary somatic embryos and embryogenic calli. These clusters were sub-cultured every 4 weeks, and an average of 10% of the secondary somatic embryos developed into plantlets in each subculture. The same culture on WPM containing Abscisic acid (ABA) in different concentrations (3.48, 7.67, 11.35 µM) also yielded de novo secondary embryos and whitish embryogenic calli. en_US
dc.language.iso en en_US
dc.publisher Karachi: Pakistan Journal of Botany, botanical garden, university of karachi en_US
dc.title PRIMARY AND SECONDARY SOMATIC EMBRYOGENESIS FROM LEAF EXPLANTS OF ROSEMARY (ROSMARINUS OFFICINALIS L. - LAMIACEAE) en_US
dc.type Article en_US


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