dc.contributor.author |
Ghufran, Hafiz |
|
dc.contributor.author |
Riaz, Sehar |
|
dc.contributor.author |
Mahmood, Nasir |
|
dc.contributor.author |
Yaqoob, Muhammad |
|
dc.contributor.author |
Shahid, Saman |
|
dc.contributor.author |
Bhinder, Munir Ahmad |
|
dc.contributor.author |
Anwar, Saadia |
|
dc.contributor.author |
Faizan, Mahwish |
|
dc.date.accessioned |
2022-12-05T09:49:18Z |
|
dc.date.available |
2022-12-05T09:49:18Z |
|
dc.date.issued |
2019-09-21 |
|
dc.identifier.citation |
Syedbasha, M. (2019). The immune modulatory role of interferon lambda on human B-cell functions (Doctoral dissertation, University_of_Basel). |
en_US |
dc.identifier.issn |
1011-601X |
|
dc.identifier.uri |
http://142.54.178.187:9060/xmlui/handle/123456789/14668 |
|
dc.description.abstract |
We conducted genotypic analyses of interleukin-10 (IL-10) (-1082 G/A; GG, GA, AA) and interleukin-28B
(CC, CT, TT) genes polymorphisms in acute lymphoblastic leukemia (ALL) pediatric patients in descriptive study to evaluate the prevalence of these mutations. In amplification refractory mutation system-PCR (ARMS-PCR), one reaction was carried out for each patient’s DNA sample. For IL-28B gene, two forward and two reverse primers specific for Callele and T-allele were used separately. For human IL-10 gene, two different forward primers specific for A and G alleles were used in combination with common reverse primer. IL-10 gene promoter showed highest frequency (n=29, 58%) of heterozygous (GA) allele, while genotypic analysis of IL-28B gene showed highest frequency (n=28, 56%) of homozygous (CC) allele. The IL-10 (AA) genotype related to its protein’s less production in body which may be associated with the least survival of ALL’s patients, while IL-28b (CT and TT) genotypes may be associated with less IFNλ3 levels and less life expectancy. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Karachi: Faculty of Pharmacy & Pharmaceutical Sciences, Karachi |
en_US |
dc.subject |
Acute lymphoblastic leukemia (ALL) |
en_US |
dc.subject |
Interleukin 10 (IL-10) |
en_US |
dc.subject |
Interleukin 28B (IL-28B) |
en_US |
dc.subject |
Single nucleotide polymorphism (SNP) |
en_US |
dc.title |
Polymorphism of Interleukin-10 (IL-10, -1082 G/A) and Interleukin28B (IL-28B, C/T) in pediatric Acute Lymphoblastic Leukemia (ALL) |
en_US |
dc.type |
Article |
en_US |