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OPTIMIZATION OF CULTURAL CONDITIONS FOR THE PRODUCTION OF ALPHA AMYLASE BY ASPERGILLUS NIGER (BTM-26) IN SOLID STATE FERMENTATION

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dc.contributor.author ABDULLAH, ROHEENA
dc.contributor.author SHAHEEN, NAEEMA
dc.contributor.author MEHWISH IQTEDAR, MEHWISH
dc.contributor.author NAZ, SHAGUFTA
dc.contributor.author IFTIKHAR, TEHREEMA
dc.date.accessioned 2022-12-06T04:36:40Z
dc.date.available 2022-12-06T04:36:40Z
dc.date.issued 2014-07-09
dc.identifier.citation Abdullah, R., Shaheen, N., Iqtedar, M., & Naz, S. (2014). Optimization of cultural conditions for the production of alpha amylase by Aspergillus Niger (BTM-26) in solid state fermentation. Pakistan Journal of Botany, 46(3), 1071-1078. en_US
dc.identifier.issn 2070-3368
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/14688
dc.description.abstract The present study deals with the isolation, screening and selection of native fungal strain for the alpha amylase production. Forty fungal strains were isolated from different soil samples. These strains were initially screened qualitatively on starch agar medium and quantitative screening was carried out in solid state fermentation. A strain of Aspergillus niger showing maximum production (432 ± 0.9 U/ml/min) of enzyme was selected and assigned the code BTM-26. The yield on various agricultural products, namely, coconut oil cake (COC), rice bran (RB), vegetable wastes or banana peel and wheat bran (WB) was compared. Wheat bran proved to be the best substrate for alpha amylase production. The effect of incubation temperature, initial pH, and inoculum size was investigated for the enzyme production. The maximum enzyme production was obtained at 30°C, pH 5, and inoculum size of 1 ml. The rate of fermentation was also studied and the highest yield of enzyme was obtained after 72 h of inoculation. Addition of 1.5% lactose as carbon source and 0.2% (NH4)2SO4 and 0.3% yeast extract as inorganic and organic nitrogen sources respectively gave enzyme production 990 ± 0.81 U/ml/min which reflects en_US
dc.language.iso en en_US
dc.publisher Karachi: Pakistan Journal of Botany , Botanical garden , University of Karachi en_US
dc.title OPTIMIZATION OF CULTURAL CONDITIONS FOR THE PRODUCTION OF ALPHA AMYLASE BY ASPERGILLUS NIGER (BTM-26) IN SOLID STATE FERMENTATION en_US
dc.type Article en_US


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