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PHENYLALANINE AMMONIA-LYASE (PAL) AND PEROXIDASE ACTIVITY IN BROWN RUST INFECTED TISSUES OF PAKISTANI WHEAT CULTIVARS

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dc.contributor.author RIAZ, ADNAN
dc.contributor.author RIAZ, ABID
dc.contributor.author RATTU, ATIQ UR REHMAN
dc.contributor.author TAHIR, MUHAMMAD IBRAHIM
dc.contributor.author AZEEM, MUHAMMAD
dc.date.accessioned 2022-12-06T05:03:37Z
dc.date.available 2022-12-06T05:03:37Z
dc.date.issued 2014-07-14
dc.identifier.citation Riaz, A., Riaz, A., Rattu, A. U. R., Tahir, M. I., & Azeem, M. (2014). Phenylalanine ammonia-lyase (PAL) and peroxidase activity in brown rust infected tissues of Pakistani wheat cultivars. Pak. J. Bot, 46(3), 1101-1107. en_US
dc.identifier.issn 2070-3368
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/14714
dc.description.abstract Besides other factors resistance and susceptibility is the outcome of biochemical processes such as activities of defenserelated enzymes. So in this study, Phenylalanine ammonia-lyase (PAL) and Peroxidase activity of resistant (Inqilab-91) and susceptible (Kirin-95) wheat cultivars were determined through spectrophotometer to address the biochemical aspect related to the disease after 8 hours, 24 hours, 48 hours and 72 hours of leaf rust inoculation. The results have shown that these enzymes were present in both the resistant and susceptible cultivars but the activity was more pronounced in the resistant one. The effect of PAL and peroxidase activity was also investigated among inoculated and uninoculated plants within the same cultivar. The activity of both PAL and peroxidase were more significant in inoculated ones. The results have shown that the after 72 hours of inoculation Inqilab-91 had more PAL activity i.e., 5.47 IU/ml/min than in Kirin-95 i.e., 2.08 IU/ml/min at 270 nm. While peroxidase activity in Inqilab-91 was 6.41 IU/ml/min and in Kirin-95, 3.66 IU/ml/min after 72 hours of inoculation, observed under 470 nm wavelength. Increase in one’s activity increases the other enzyme’s activity. The activity was more prominent after 72 hours of infection as pathogen had successfully established itself in the host plant tissue. The activities of these enzymes act as plants active defense mechanism against the attack of pathogen en_US
dc.language.iso en en_US
dc.publisher Karachi: Pakistan Journal of Botany , Botanical garden , University of Karachi en_US
dc.title PHENYLALANINE AMMONIA-LYASE (PAL) AND PEROXIDASE ACTIVITY IN BROWN RUST INFECTED TISSUES OF PAKISTANI WHEAT CULTIVARS en_US
dc.type Article en_US


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