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RANDOM MUTAGENESIS OF ASPERGILLUS NIGER AND PROCESS OPTIMIZATION FOR ENHANCED PRODUCTION OF GLUCOSE OXIDASE

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dc.contributor.author IKRAM UL HAQ, IKRAM UL HAQ
dc.contributor.author NAWAZ, ALI
dc.contributor.author MUKHTAR, HAMID
dc.contributor.author MANSOOR, ZINNIA
dc.contributor.author RIAZ, MARIYUM
dc.contributor.author AHMED, MADEEHA
dc.contributor.author AMEER, SYED MUHAMMAD
dc.date.accessioned 2022-12-06T05:08:24Z
dc.date.available 2022-12-06T05:08:24Z
dc.date.issued 2014-07-15
dc.identifier.citation Haq, I. U., Nawaz, A., Mukhtar, H., Mansoor, Z., Riaz, M., Ahmed, M., & Ameer, S. M. (2014). Random mutagenesis of Aspergillus niger and process optimization for enhanced production of glucose oxidase. Pak. J. Bot, 46(3), 1109-1114. en_US
dc.identifier.issn 2070-3368
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/14717
dc.description.abstract The study deals with the improvement of wild strain Aspergillus niger IIB-31 through random mutagenesis using chemical mutagens. The main aim of the work was to enhance the glucose oxidase (GOX) yield of wild strain (24.57±0.01 U/g of cell mass) through random mutagenesis and process optimization. The wild strain of Aspergillus niger IIB-31 was treated with chemical mutagens such as Ethyl methane sulphonate (EMS) and nitrous acid for this purpose. Mutagen treated 98 variants indicating the positive results were picked and screened for the glucose oxidase production using submerged fermentation. EMS treated E45 mutant strain gave the highest glucose oxidase production (69.47 + 0.01 U/g of cell mass), which was approximately 3-folds greater than the wild strain IIB-31. The preliminary cultural conditions for the production of glucose oxidase using submerged fermentation from strain E45 were also optimized. The highest yield of GOD was obtained using 8% glucose as carbon and 0.3% peptone as nitrogen source at a medium pH of 7.0 after an incubation period of 72 hrs at 30ºC en_US
dc.language.iso en en_US
dc.publisher Karachi: Pakistan Journal of Botany , Botanical garden , University of Karachi en_US
dc.title RANDOM MUTAGENESIS OF ASPERGILLUS NIGER AND PROCESS OPTIMIZATION FOR ENHANCED PRODUCTION OF GLUCOSE OXIDASE en_US
dc.type Article en_US


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