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Simultaneous identification of the three active constituents in LungVentilating-Regulating Oral Liquid by RP-HPLC

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dc.contributor.author Lu, Lina
dc.contributor.author Zuo, Yanhua
dc.contributor.author Wang, Yaoxia
dc.contributor.author Shi, Yongqing
dc.contributor.author Kang, Shuhe
dc.contributor.author Luo, Xingping
dc.contributor.author Liang, Zuochong
dc.contributor.author Chen, Lihua
dc.date.accessioned 2022-12-06T05:59:07Z
dc.date.available 2022-12-06T05:59:07Z
dc.date.issued 2019-09-20
dc.identifier.citation Lu, L., Zuo, Y., Wang, Y., Shi, Y., Kang, S., Luo, X., ... & Chen, L. (2019). Simultaneous identification of the three active constituents in Lung-Ventilating-Regulating Oral Liquid by RP-HPLC. Pakistan Journal of Pharmaceutical Sciences, 32. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/14734
dc.description.abstract The study aimed to develop a HPLC method for ephedrine, hesperidin, and baicalin in Lung-VentilatingRegulating Oral Liquid. The three active constituents were identified in an Agilent TC-C18 (2) chromatographic column (250mm × 4.6mm, 5μm), with 0.2% phosphoric acid solution - methyl cyanides as mobile phase, which was performed at a gradient elution column temperature of 25oC and a flow rate of 0.8 mL•min-1. Then the eluate was detected at detection wavelengths of 207 nm (for ephedrine) and 278 nm (for hesperidin and baicalin). Under the chromatographic conditions, ephedrine, hesperidin, and baicalin were well separated, which showed good linear relationships at 0.1582.370, 0.164-4.100 and 0.160-4.000µg, respectively. The coefficients of recovery of these three kinds of samples showed 100.2%, 98.7% and 97.8%, respectively. The developed method is convenient, accurate and well repeatable, and consequently can be applied for the quality control of Lung-Ventilating-Regulating Oral Liquid en_US
dc.language.iso en en_US
dc.publisher Karachi:Pakistan Journal of Pharmaceutical Sciences, university of Karachi. en_US
dc.subject Baicalin en_US
dc.subject ephedrine en_US
dc.subject hesperidin en_US
dc.subject hplc en_US
dc.subject chromatographic conditions en_US
dc.subject phosphoric acid solution en_US
dc.title Simultaneous identification of the three active constituents in LungVentilating-Regulating Oral Liquid by RP-HPLC en_US
dc.type Article en_US


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