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A COMPARISON OF SIX TOTAL RNA ISOLATION METHODS FOR DIAGNOSIS OF GYSVD-1 (GRAPEVINE YELLOW SPECKLE VIROID-1) ON VITIS VINIFERA L. LEAVES

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dc.contributor.author PAYLAN, İSMAIL CAN
dc.contributor.author ÇANDAR, AYŞE
dc.contributor.author GÜMÜŞ, MUSTAFA
dc.contributor.author PAZARLAR, SERCAN
dc.contributor.author ÖNDER, SERKAN
dc.date.accessioned 2022-12-06T06:14:57Z
dc.date.available 2022-12-06T06:14:57Z
dc.date.issued 2014-12-22
dc.identifier.citation Paylan, I. C., ÇANDAR, A., Gümüş, M., Pazarlar, S., & Önder, S. E. R. K. A. N. (2014). A comparison of six total rna isolation methods for diagnosis of gysvd-1 (grapevine yellow speckle viroid-1) on vitis vinifera L. leaves. Pakistan Journal of Botany, 46(6), 2089-2093. en_US
dc.identifier.issn 2070-3368
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/14739
dc.description.abstract Isolation of high quality RNA from plant tissues is one of the most critical steps for the successful application of Vdiagnostic tests such as reverse transcription polymerase chain reaction (RT-PCR), northern blotting, microarray hybridization. The presence of inhibitors such as secondary metabolites, phenolic compounds and RNAses can cause inaccurate and undesirable results. Grapevine is rich in a wide range of metabolites which interfere with RNA isolation. From this point of view, we researched six different total RNA extraction methods on leaves of Vitisvinifera L. to find the best one that contribute the purity and high quality. The methods tested are silica-capture, modified silica-capture, commercial kit, the new combined, lithium chloride and citric buffer. RNA quality was analyzedspectrophotometrically by nanodrop, agarose gel electrophoresis and RT-PCR. As a result of all, it is clear that the most suitable TNA isolation protocol is the new combined method which experienced and named firstly by us, in terms of RNA purity, concentration, less time consuming of isolation step and achievement on detection of GYSVd-1. en_US
dc.language.iso en en_US
dc.publisher Karachi: Pakistan Journal of Botany , Botanical garden , University of Karachi en_US
dc.subject Grapevine en_US
dc.subject GYSVd-1 en_US
dc.subject RNA isolation en_US
dc.subject TNA extraction methods en_US
dc.subject Viroid diagnosis en_US
dc.title A COMPARISON OF SIX TOTAL RNA ISOLATION METHODS FOR DIAGNOSIS OF GYSVD-1 (GRAPEVINE YELLOW SPECKLE VIROID-1) ON VITIS VINIFERA L. LEAVES en_US
dc.type Article en_US


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