PASTIC Dspace Repository

Propofol regulates imbalanced Th17/Treg responses in lipopolysaccharide-induced septic shock rats

Show simple item record

dc.contributor.author Xie, Lei
dc.contributor.author Huang, Li-jun
dc.contributor.author Wang, Sheng-you
dc.contributor.author Fang, Xiangdong
dc.date.accessioned 2022-12-06T06:31:37Z
dc.date.available 2022-12-06T06:31:37Z
dc.date.issued 2019-09-20
dc.identifier.citation Xie, L., Huang, L. J., Wang, S. Y., & Fang, X. (2019). Propofol regulates imbalanced Th17/Treg responses in lipopolysaccharide-induced septic shock rats. Pakistan Journal of Pharmaceutical Sciences, 32. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/14742
dc.description.abstract Propofol (PPF) has previously been shown to inhibit the inflammatory response to septic shock. The main purpose of the present study was to investigate the effects of PPF on the levels of regulatory T cells (Treg) and Th17 in septic shock. Septic shock in rats was induced by intraperitoneal injection of lipopolysaccharide (LPS), and PPF (100 mg/kg) was administered. Mortality, the mean arterial pressure (MAP) and heart rates (HR) were recorded for 24 h after LPS injection. The Treg and Th17 ratios were analysed by flow cytometry. Moreover, the expression of p-STAT3, pSTAT5, STAT3, and STAT5 in PBMCs was measured by western blotting. The results showed that the MAP and HR of the PPF group were more stable than those of the LPS group. Mortality at 24 h after LPS injection was much lower in the PPF group compared to that in the LPS group. PPF significantly reduced the levels of IL-17, TNF-α and IL-6 but increased the IL-10 concentration. Moreover, PPF-treated rats exhibited a higher level of circulating Treg cells and a lower level of circulating Th17 cells in comparison to untreated rats. PPF decreased the level of phosphorylated STAT3 (p-STAT3), increased the level of p-STAT5, but did not change the levels of STAT3 and STAT5. Our data suggest that PPF regulates the imbalanced level of Th17/Treg in septic rats, possibly through modulating the expression of p-STAT3 and p-STAT5. en_US
dc.language.iso en en_US
dc.publisher Karachi:Pakistan Journal of Pharmaceutical Sciences, university of Karachi. en_US
dc.subject Propofol en_US
dc.subject septic shock en_US
dc.subject lipopolysaccharide en_US
dc.subject Treg, Th17 en_US
dc.title Propofol regulates imbalanced Th17/Treg responses in lipopolysaccharide-induced septic shock rats en_US
dc.type Article en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account