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ASSESSMENT OF GENETIC VARIABILITY IN SOMACLONAL POPULATION OF SUGARCANE

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dc.contributor.author SEEMA, NIGHAT
dc.contributor.author KHAN, IMTIAZ AHMED
dc.contributor.author RAZA, SABOOHI
dc.contributor.author YASMEEN, SHAFQUAT
dc.contributor.author BIBI, SAJIDA
dc.contributor.author NIZAMANI, GHULAM SHAH
dc.date.accessioned 2022-12-06T06:39:54Z
dc.date.available 2022-12-06T06:39:54Z
dc.date.issued 2014-12-25
dc.identifier.citation ASSESSMENT OF GENETIC VARIABILITY IN SOMACLONAL POPULATION OF SUGARCANE en_US
dc.identifier.issn 2070-3368
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/14744
dc.description.abstract In the present study plant tissue culture technique was used to create the genetic variability in three sugarcane clones NIA98, BL4 and AEC82-1026. Callus induced in these clones in media containing MS + 2, 4 D (2mg 1it-1) and Dicamba (1mg1it-1). The embryogenic calli then regenerated in media containing MS basal media + Kinetin (2mg1it-1) + IBA (2mg1it-1) + IAA (2mglit-1). After shooting and rooting, plants were exposed to green house and acclimatization of the somaclones in the field condition. RAPD markers were used to evaluate the genetic variation at DNA level between parents and somaclones of NIA98, BL4 and AEC82-1026 developed through callus culture. Fourteen RAPD primer chosen randomly were used to amplify DNA from plant material to assess the genetic variation between parents and regenerated somaclones. The highest similarity was obtained between BL4 parent and BL4 somaclone (96%). While minimum similarity found between NIA-98 parent and AEC82-1026 somaclone (69%). In this study, we used RAPD to investigate the somaclonal variation in sugarcane clones derived from callus cultures. en_US
dc.language.iso en en_US
dc.publisher Karachi: Pakistan Journal of Botany , Botanical garden , University of Karachi en_US
dc.subject Genetic variability en_US
dc.subject Somaclone en_US
dc.subject Sugarcane en_US
dc.title ASSESSMENT OF GENETIC VARIABILITY IN SOMACLONAL POPULATION OF SUGARCANE en_US
dc.type Article en_US


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