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ASSESSMENT OF SILVER NITRATE ON CALLUS INDUCTION AND IN VITRO SHOOT REGENERATION IN TOMATO (SOLANUM LYCOPERSICUM MILL.)

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dc.contributor.author SHAH, SABIR HUSSAIN
dc.contributor.author SHAUKAT ALI
dc.contributor.author JAN, SOHAIL AHMAD
dc.contributor.author UD–DIN, JALAL
dc.contributor.author ALI, GHULAM MUHAMMAD
dc.date.accessioned 2022-12-07T03:14:32Z
dc.date.available 2022-12-07T03:14:32Z
dc.date.issued 2015-01-01
dc.identifier.citation Shah, S. H., Ali, S., Jan, S. A., Din, J., & Ali, G. M. (2014). Assessment of silver nitrate on callus induction and in vitro shoot regeneration in tomato (Solanum lycopersicum Mill.). Pakistan Journal of Botany, 46(6), 2163-2172. en_US
dc.identifier.issn 2070-3368
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/14773
dc.description.abstract In vitro morphogenesis is greatly influenced by a gaseous plant growth regulator (ethylene). The effect of silver nitrate (AgNO3) and different plant growth regulators were assessed on callus induction, In vitro shoot regeneration and multiple primordial shoots per explant in tomato. The maximum callus induction frequency was recorded culturing hypocotyls, while In vitro shoot regeneration frequency and the number of primordial shoots per explant were significantly higher when leaf discs were used as explants. The callus induction frequency was improved by the supplementation of 10-15 mg/l AgNO3 in MS basal media along with 2.0 mg/l IAA, 2.5 mg/l BAP and yielded the highest callus induction frequency (91.33%) in cv. Rio Grande, followed by Roma (88.33%) and Moneymaker (82.66%). Similarly, the highest In vitro shoot regeneration frequency (96.66, 92.66 and 90%) was recorded in Rio Grande, Roma and Moneymaker on MS media fortified with 0.1 mg/l IAA, 1.0 mg/l ZEA and 2.0 mg/l BAP along with 8-10 mg/l AgNO3. AgNO3 also had promotive effect on induction of multiple shoots per explant. These findings indicate that ethylene is linked with the suppression of In vitro morphogenesis in tomato and AgNO3 interacts with ethylene and enhances callus induction and In vitro shoot regeneration in tomato. en_US
dc.language.iso en en_US
dc.publisher Karachi: Pakistan Journal of Botany, Botanical Garden, University of Karachi en_US
dc.subject Ethylene inhibitor en_US
dc.subject Auxins en_US
dc.subject Cytokinins; Hypocotyls en_US
dc.subject Leaf discs en_US
dc.subject Callus induction en_US
dc.subject In vitro shoot regeneration en_US
dc.subject Solanum lycopersicum en_US
dc.title ASSESSMENT OF SILVER NITRATE ON CALLUS INDUCTION AND IN VITRO SHOOT REGENERATION IN TOMATO (SOLANUM LYCOPERSICUM MILL.) en_US
dc.type Article en_US


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