Anti-inflammatory effect of austroyunnane B on RAW264.7 cells

Abstract

Austroyunnane B, a highly oxidized guaianolide-type sesquiterpenoid isolated from the whole plants of Artemisia austro-yunnanensis by our group, revealed significant inhibition against NO production on the model of lipopolysaccharide (LPS)-stimulated RAW264.7 cells. In this study, its anti-inflammatory effect on some inflammatory cytokines and proteins was further evaluated using the same model. As results, co-treatment with different concentrations (0.39-100μmol /L) of austroyunnane B on LPS-induced RAW264.7 cells displayed reduction of the nitric oxide (NO) generation in a dose dependent manner. It also showed a concentration-dependent inhibition against TNF-α, IL-1β, IL-6 and IL-10, and down-regulation of protein expressions of iNOS and COX-2 on that model at the range of 0.625-10μmol/L, suggesting that austroyunnane B can be used as a lead compound for the development of inflammatory drug.