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CHARACTERIZATION OF THE CAUSAL ORGANISM OF BLACKLEG AND SOFT ROT OF POTATO, AND MANAGEMENT OF THE DISEASE WITH BALANCED FERTILIZATION

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dc.contributor.author ALI, HAFIZ FARHAD
dc.contributor.author BIBI, AYESHA
dc.contributor.author AHMAD, MUSHARAF
dc.contributor.author JUNAID, MUHAMMAD
dc.contributor.author ALI, ASAD
dc.contributor.author HUSSAIN, SHAUKAT
dc.contributor.author ALAM, SHAH
dc.contributor.author ALAM, SARTAJ
dc.date.accessioned 2022-12-07T05:16:40Z
dc.date.available 2022-12-07T05:16:40Z
dc.date.issued 2015-01-15
dc.identifier.citation Ali, H. F., Bibi, A., Ahmad, M., Junaid, M., Ali, A., Hussain, S., ... & Alam, S. (2014). Characterization of the causal organism of blackleg and soft rot of potato and management of the disease with balanced fertilization. Pak. J. Bot, 46(6), 2277-2284. en_US
dc.identifier.issn 2070-3368
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/14809
dc.description.abstract Based upon colony morphology, physio-biochemical tests and polymerase chain reaction (using species or subspeciesspecific primers) studies, 20 isolates (out of a total of 42) were found to be Erwinia carotovora subspecies atroseptica (Eca), 19 were identified as Erwinia carotovora subspecies carotovora (Ecc), and 3 as Erwinia chrysanthemi (Ech). Results of the subspecies-differentiating biochemical tests indicated that majority of the candidate Ecc isolates did not produce acid from α-methyle glucoside (as expected) but their reaction to the production of reducing substances from sucrose was variable. Likewise, some of our Eca and Ecc strains (unexpectedly) were sensitive to erythromycin. Also, most of our Eca strains unexpectedly grew at 36o C. Our strains slightly deviate from the standard description in some of their minor characteristics but they still remain the valid members of the Eca, Ecc or Ech group as similar variations in minor characteristics have been found by other workers. The occurrence of intermediate forms of Eca and Ecc (sharing some of the characteristics of both the groups) indicates variability happening among these strains. This variability indicates the potential ability of the pathogen to break the resistance of the host. The results of the effect of balanced nutrition in controlling blackleg and soft rot of potatoes indicated that the fertilizer combination of N3P1K3 (262/252/262 kg.ha-1) which is slightly higher than the normally practiced dose (247/247/247 kg.ha-1) was the best in bringing the disease to a minimum and subsequently increasing the yield en_US
dc.language.iso en en_US
dc.publisher Karachi: Pakistan Journal of Botany , Botanical garden , University of Karachi en_US
dc.subject Erwinia carotovora subsp en_US
dc.subject atroseptica en_US
dc.subject Eca en_US
dc.subject Blackleg en_US
dc.subject PCR en_US
dc.subject isolate en_US
dc.title CHARACTERIZATION OF THE CAUSAL ORGANISM OF BLACKLEG AND SOFT ROT OF POTATO, AND MANAGEMENT OF THE DISEASE WITH BALANCED FERTILIZATION en_US
dc.type Article en_US


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