dc.contributor.author |
ZHAO, LINGLING |
|
dc.contributor.author |
DIAO, YING |
|
dc.contributor.author |
JIN, SURONG |
|
dc.contributor.author |
ZHOU, FASONG |
|
dc.contributor.author |
HU, ZHONGLI |
|
dc.date.accessioned |
2022-12-09T06:04:31Z |
|
dc.date.available |
2022-12-09T06:04:31Z |
|
dc.date.issued |
2016-05-10 |
|
dc.identifier.citation |
Zhao, L., Diao, Y., Jin, S., Zhou, F., & Hu, Z. (2016). Effect of genotype and inflorescence developmental stage on callus induction and plant regeneration of Miscanthus lutarioripariu, a non-food biomass crop. Pakistan Journal of Botany, 48(2), 717-722. |
en_US |
dc.identifier.issn |
2070-3368 |
|
dc.identifier.uri |
http://142.54.178.187:9060/xmlui/handle/123456789/14889 |
|
dc.description.abstract |
Miscanthus lutarioriparius, is a perennial C4 weeds. In this study, tissue culture responses were evaluated in four genotyoes of M. lutarioriparius which collected from the Yangtze River basin. A total of four types of callus with different characteristics and induction frequencies were induced from four genotypes immature inflorescences on medium Murashige and Skoog (MS) containing 4.0 mg/L 2,4-dichlorophenoxyacetic acid (2, 4-D). Only ML04 generated the embryogenic callus. According to the length of immature inflorescences, they divided into three inflorescences developmental stages which generated different induction percentages of embryogenic callus. Developmental stage B with 50-100 mm length inflorescence generated the highest induction percentages of embryogenic callus. The regeneration rate was 65.56% (4.0 mg/L kinetin), 91.50% (5.0 mg/L KT) and 26.67% (6.0 mg/L KT) from embryogenic callus, respectively. The shoots were transferred onto the Murashige and Skoog (MS) basal medium for rooting, the rooting rate was 100%. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Karachi: Pakistan Journal of Botany , Botanical garden , University of Karachi |
en_US |
dc.subject |
Micanthus lutarioripariu |
en_US |
dc.subject |
nflorescences developmental stage |
en_US |
dc.subject |
Embryogenic callus |
en_US |
dc.subject |
Plant regeneration |
en_US |
dc.title |
EFFECT OF GENOTYPE AND INFLORESCENCE DEVELOPMENTAL STAGE ON CALLUS INDUCTION AND PLANT REGENERATION OF MISCANTHUS LUTARIORIPARIU, A NON-FOOD BIOMASS CROP |
en_US |
dc.type |
Article |
en_US |