Anti-Candida and antioxidant activities of hydroalcohlic extract of Rumex obtusifolius leaves

Abstract

Due to increasing the use of antifungal drugs, the development of resistance in some Candida species and the consumption of the side effects of chemical drugs, use of new resources, especially medicinal plants are very important. The aim of this study was to investigate of anti-Candida and antioxidant activities of hydroalcohlic extract from leaves of Rumex obtusifolius. The Rumex obtusifolius Leaves were extracted using Ethyl acetate; methanol and distilled water (6:3:1) by Sox helet system. The hydroalcoholic extraction of Rumex obtusifolius was evaluated for their antioxidant capacities using in vitro methods; including 1-diphenyl-2-picrylhydrazyl radical scavenging, β-Carotene bleaching test and reducing power assay. Total free phenolics, total flavonoids content and as well as the antifungal activity were also examined. The components of extract were analyzed via GC-Mass instrument. The extract was screened against 40 isolated pathogenic Candida species such as C. albicans and C. glabrata through agar diffusion method. The hydroalcoholic extract can strongly scavenge DPPH radical and its antioxidant capacities which are high correlated with the total free Phenolics and total flavonoids. Also, the extract had high capability inhibition of linoleic acid oxidation and the reducing ability. This study revealed a higher antioxidant capacity in the leaves of Rumex obtusifolius compared with control groups. The minimum inhibitory concentration values within 24 and 48 hours were 200-250µg/µL for C. albicans and 250µg/µL for C. glabrata. The extract includes high amounts of phenolic compounds and antioxidant activity showing is significant. Also, the results confirmed that leaves extract had a potential in anti-Candida activity and suggesting that it could be utilized as a potential source of herbal medicine drugs and natural antioxidants to prevent diseases associated with free radical, anti-fungal disease and food preservation.