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HPLC-UV method for simultaneous determination of sparfloxacin and dexamethasone sodium phosphate in eye drops

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dc.contributor.author Prakash, Katakam
dc.contributor.author Sireesha, Karanam R
dc.date.accessioned 2022-12-13T04:39:41Z
dc.date.available 2022-12-13T04:39:41Z
dc.date.issued 2019-05-24
dc.identifier.citation Prakash, K., & Sireesha, K. R. (2019). HPLC-UV method for simultaneous determination of sparfloxacin and dexamethasone sodium phosphate in eye drops. Pak J Pharm Sci, 32, 1057-61. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/14947
dc.description.abstract A simple, sensitive liquid chromatographic method was developed and validated for the simultaneous estimation of sparfloxacin and dexamethasone sodium phosphate in bulk and pharmaceutical formulations. Optimum separation was achieved in less than 10 min using a C18 column (250 mmx4.6 mm i.d, 5µ particle size) by isocratic elution. The mobile phase consisting of a mixture of mixed phosphate buffer (pH 6.8) and acetonitrile (50:50, v/v) was used. Column effluents were monitored at 224nm at a flow rate of 1ml/min. Retention times of sparfloxacin and dexamethasone sodium phosphate were 3.01 and 6.47 min respectively. The linearity of sparfloxacin and dexamethasone sodium phosphate was in the range of 3-18µg/ml and 1-6µg/ml respectively. Developed method was economical because, the time taken and amount of solvent consumed for each analysis was less. The method was validated and was applied to the simultaneous determination of sparfloxacin and dexamethasone sodium phosphate in bulk and pharmaceutical formulations. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Simultaneous determination en_US
dc.subject HPLC en_US
dc.subject isocratic elution en_US
dc.subject validation en_US
dc.title HPLC-UV method for simultaneous determination of sparfloxacin and dexamethasone sodium phosphate in eye drops en_US
dc.type Article en_US


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