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Glucagon-like peptide-1 attenuates liver injury in the rat diabetes model

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dc.contributor.author Yang, Zhenxing
dc.contributor.author Guo, Caiyun
dc.contributor.author Ma, Jun
dc.contributor.author Lu, Yang
dc.contributor.author Xuan, Liping
dc.contributor.author Huang, Yongmin
dc.contributor.author Yang, Xiaoyu
dc.contributor.author Zhao, Minxia
dc.contributor.author Luo, Yaner
dc.date.accessioned 2022-12-13T09:54:56Z
dc.date.available 2022-12-13T09:54:56Z
dc.date.issued 2019-01-10
dc.identifier.citation Yang, Z., Guo, C., Ma, J., Lu, Y., Xuan, L., Huang, Y., ... & Luo, Y. (2019). Glucagon-like peptide-1 attenuates liver injury in the rat diabetes model. Pakistan Journal of Pharmaceutical Sciences, 32. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/15001
dc.description.abstract The aim of present study is to examine the effect of glucagon-like peptide-1(GLP-1) on diabetes-induced liver injury and explore detailed mechanisms of GLP-1 hepatoprotective effect. 150 male Sprague-Dawley rats were randomly assigned into three groups with equal number, including Sham group, diabetes group and GLP-1 intervention group. Diabetes rat model was performed with intraperitoneal injection of streptozotocin (STZ, 65mg/kg). Fasting bloodglucose of rat model was assessed at 72h after STZ injection to verify diabetes rat model. Rats in Sham group were normally fed. Rats in GLP-1 intervention group received 2 ng/kg GLP-1 intervention, at 2, 4, 6 and 8 weeks after intervention, TUNEL staining were performed to examine apoptosis of liver tissue. PCR and Western blot were performed to examine insulin, GLP-1R, autophagy-associated gene and HDAC-1. Compared with diabetes group, insulin expression of GLP-1 intervention group increased significantly (P<0.05). TUNEL staining at different time showed apoptosis levels of liver tissues were reduced gradually after GLP-1 intervention (P<0.05). Compared with diabetes groups, the expressions of BCL2 and GLP-1R were increased, while the levels of caspase3 and LC3 were reduced in GLP-1 intervention group (P<0.05). GLP-1 treatment decreased levels of phosphorylated AKT, phosphorylated ERK1/2, and HDAC6 in liver tissues (P<0.05). GLP-1 treatment alleviated diabetes-induced liver injury via regulating autophagy. The mechanism of GLP-1 hepatoprotective effect could be via GLP-1R-ERK1/2-HDAC6 signaling pathway. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Diabetes en_US
dc.subject liver injury en_US
dc.subject glucagon-like peptide en_US
dc.subject autophagy en_US
dc.title Glucagon-like peptide-1 attenuates liver injury in the rat diabetes model en_US
dc.type Article en_US


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