dc.contributor.author |
Yang, Zhenxing |
|
dc.contributor.author |
Guo, Caiyun |
|
dc.contributor.author |
Ma, Jun |
|
dc.contributor.author |
Lu, Yang |
|
dc.contributor.author |
Xuan, Liping |
|
dc.contributor.author |
Huang, Yongmin |
|
dc.contributor.author |
Yang, Xiaoyu |
|
dc.contributor.author |
Zhao, Minxia |
|
dc.contributor.author |
Luo, Yaner |
|
dc.date.accessioned |
2022-12-13T09:54:56Z |
|
dc.date.available |
2022-12-13T09:54:56Z |
|
dc.date.issued |
2019-01-10 |
|
dc.identifier.citation |
Yang, Z., Guo, C., Ma, J., Lu, Y., Xuan, L., Huang, Y., ... & Luo, Y. (2019). Glucagon-like peptide-1 attenuates liver injury in the rat diabetes model. Pakistan Journal of Pharmaceutical Sciences, 32. |
en_US |
dc.identifier.issn |
1011-601X |
|
dc.identifier.uri |
http://142.54.178.187:9060/xmlui/handle/123456789/15001 |
|
dc.description.abstract |
The aim of present study is to examine the effect of glucagon-like peptide-1(GLP-1) on diabetes-induced liver
injury and explore detailed mechanisms of GLP-1 hepatoprotective effect. 150 male Sprague-Dawley rats were randomly assigned into three groups with equal number, including Sham group, diabetes group and GLP-1 intervention group. Diabetes rat model was performed with intraperitoneal injection of streptozotocin (STZ, 65mg/kg). Fasting bloodglucose of rat model was assessed at 72h after STZ injection to verify diabetes rat model. Rats in Sham group were normally fed. Rats in GLP-1 intervention group received 2 ng/kg GLP-1 intervention, at 2, 4, 6 and 8 weeks after intervention, TUNEL staining were performed to examine apoptosis of liver tissue. PCR and Western blot were performed to examine insulin, GLP-1R, autophagy-associated gene and HDAC-1. Compared with diabetes group, insulin expression of GLP-1 intervention group increased significantly (P<0.05). TUNEL staining at different time showed apoptosis levels of liver tissues were reduced gradually after GLP-1 intervention (P<0.05). Compared with diabetes groups, the expressions of BCL2 and GLP-1R were increased, while the levels of caspase3 and LC3 were reduced in GLP-1 intervention group (P<0.05). GLP-1 treatment decreased levels of phosphorylated AKT, phosphorylated ERK1/2, and HDAC6 in liver tissues (P<0.05). GLP-1 treatment alleviated diabetes-induced liver injury via regulating autophagy.
The mechanism of GLP-1 hepatoprotective effect could be via GLP-1R-ERK1/2-HDAC6 signaling pathway. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi |
en_US |
dc.subject |
Diabetes |
en_US |
dc.subject |
liver injury |
en_US |
dc.subject |
glucagon-like peptide |
en_US |
dc.subject |
autophagy |
en_US |
dc.title |
Glucagon-like peptide-1 attenuates liver injury in the rat diabetes model |
en_US |
dc.type |
Article |
en_US |