dc.contributor.author |
Nisa, Zeb-un |
|
dc.contributor.author |
Ali, Syed Imran |
|
dc.contributor.author |
Rizvi, Mehwish |
|
dc.contributor.author |
Ahmed Khan, Maqsood |
|
dc.contributor.author |
Akhter Sultan, Rafi |
|
dc.contributor.author |
Fatima, Rasheeda |
|
dc.contributor.author |
Shaheen, Najma |
|
dc.contributor.author |
Zafar, Farya |
|
dc.contributor.author |
Kashif, Sadia Suri |
|
dc.contributor.author |
Khatian, Najeeb |
|
dc.date.accessioned |
2022-12-15T04:16:55Z |
|
dc.date.available |
2022-12-15T04:16:55Z |
|
dc.date.issued |
2019-03-20 |
|
dc.identifier.citation |
Fatima, R., Shaheen, N., Zafar, F., Kashif, S. S., & Khatian, N. (2019). Development and validation of reverse phase HPLC method for determination of angiotensin receptor blocking agent irbesartan in plasma. Pak. J. Pharm. Sci, 32(2), 853-858. |
en_US |
dc.identifier.issn |
1011-601X |
|
dc.identifier.uri |
http://142.54.178.187:9060/xmlui/handle/123456789/15101 |
|
dc.description.abstract |
A sensitive, reproducible and modest analytical procedure was developed and validated for evaluation of irbesartan in human plasma. LLE (Liquid-Liquid extraction) of the drug was carried out with acetonitrile (1:1 v/v). Chromatographic separation of irbesartan was conducted by the help of 4.0mm × 25cm column having L1 packing from plasma and mobile phase utilizing HPLC. The mobile phase comprise of phosphate buffer and acetonitrile in a ratio of 67:33 v/v. The flow rate was set at 1ml/minute and the detector at a wavelength of 220 nm. The resolution of irbesartan was well performed from plasma components. This method was validated and demonstrated linearity with a concentration range of 0.1to 6µg/ml of irbesartan in plasma. Intra-day, inter-day accuracy was found 89.33% to 96.37% while intra-day, inter-day precision was found within the limit of 0.02 and 2.15 respectively. The mean recovery of irbesartan was 97.28%. The efficacy of extraction was proved by above-mentioned results. In plasma, the 0.05 and 0.1µg/ml dilutions were exhibited as the LOD and LOQ of irbesartan. Stability studies disclosed that irbesartan showed stability at -20oC storage. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Karachi:Pakistan Journal of Pharmaceutical Sciences, university of Karachi. |
en_US |
dc.subject |
Irbesartan |
en_US |
dc.subject |
liquid–liquid extraction |
en_US |
dc.subject |
human plasma |
en_US |
dc.subject |
HPLC, validation |
en_US |
dc.title |
Development and validation of reverse phase HPLC method for determination of angiotensin receptor blocking agent irbesartan in plasma |
en_US |
dc.type |
Article |
en_US |