PASTIC Dspace Repository

Development and validation of reverse phase HPLC method for determination of angiotensin receptor blocking agent irbesartan in plasma

Show simple item record

dc.contributor.author Nisa, Zeb-un
dc.contributor.author Ali, Syed Imran
dc.contributor.author Rizvi, Mehwish
dc.contributor.author Ahmed Khan, Maqsood
dc.contributor.author Akhter Sultan, Rafi
dc.contributor.author Fatima, Rasheeda
dc.contributor.author Shaheen, Najma
dc.contributor.author Zafar, Farya
dc.contributor.author Kashif, Sadia Suri
dc.contributor.author Khatian, Najeeb
dc.date.accessioned 2022-12-15T04:16:55Z
dc.date.available 2022-12-15T04:16:55Z
dc.date.issued 2019-03-20
dc.identifier.citation Fatima, R., Shaheen, N., Zafar, F., Kashif, S. S., & Khatian, N. (2019). Development and validation of reverse phase HPLC method for determination of angiotensin receptor blocking agent irbesartan in plasma. Pak. J. Pharm. Sci, 32(2), 853-858. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/15101
dc.description.abstract A sensitive, reproducible and modest analytical procedure was developed and validated for evaluation of irbesartan in human plasma. LLE (Liquid-Liquid extraction) of the drug was carried out with acetonitrile (1:1 v/v). Chromatographic separation of irbesartan was conducted by the help of 4.0mm × 25cm column having L1 packing from plasma and mobile phase utilizing HPLC. The mobile phase comprise of phosphate buffer and acetonitrile in a ratio of 67:33 v/v. The flow rate was set at 1ml/minute and the detector at a wavelength of 220 nm. The resolution of irbesartan was well performed from plasma components. This method was validated and demonstrated linearity with a concentration range of 0.1to 6µg/ml of irbesartan in plasma. Intra-day, inter-day accuracy was found 89.33% to 96.37% while intra-day, inter-day precision was found within the limit of 0.02 and 2.15 respectively. The mean recovery of irbesartan was 97.28%. The efficacy of extraction was proved by above-mentioned results. In plasma, the 0.05 and 0.1µg/ml dilutions were exhibited as the LOD and LOQ of irbesartan. Stability studies disclosed that irbesartan showed stability at -20oC storage. en_US
dc.language.iso en en_US
dc.publisher Karachi:Pakistan Journal of Pharmaceutical Sciences, university of Karachi. en_US
dc.subject Irbesartan en_US
dc.subject liquid–liquid extraction en_US
dc.subject human plasma en_US
dc.subject HPLC, validation en_US
dc.title Development and validation of reverse phase HPLC method for determination of angiotensin receptor blocking agent irbesartan in plasma en_US
dc.type Article en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account