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Frequency of multi drug resistant Pseudomonas aeruginosa in different wound types of hospitalized patients

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dc.contributor.author Ijaz, Muhammad
dc.contributor.author Siddique, Abu Baker
dc.contributor.author Hidayat Rasool, Muhammad
dc.contributor.author Shafique, Muhammad
dc.date.accessioned 2022-12-15T04:37:02Z
dc.date.available 2022-12-15T04:37:02Z
dc.date.issued 2019-03-20
dc.identifier.citation Ijaz, M., Siddique, A. B., Rasool, M. H., & Shafique, M. (2019). Frequency of multi drug resistant Pseudomonas aeruginosa in different wound types of hospitalized patients. Pakistan Journal of Pharmaceutical Sciences, 32. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/15109
dc.description.abstract Pseudomonas aeruginosa colonization is one of the major complications of wound infection leading to higher risk of morbidity and mortality. The trend of antibiotic resistant against Pseudomonas aeruginosa is increasing day by day due to irregular and extensive use of antibiotics. The main aim of this cross- sectional study is to detect the frequency of MDR Pseudomonas aeruginosa among various types of wounds during January to December 2018. In this study total 532 clinical samples were collected from wounded patients and subjected to the isolation and identification of Pseudomonas aeruginosa by standard microbiological techniques. Molecular identification of the isolates was done through PCR by using specific primers against Oprl, OprL and PA-SS genes of Pseudomonas aeruginosa. Antibiotic susceptibility testing and minimum inhibitory concentration was done by disc diffusion method and broth dilution assay respectively. PCR was performed for the molecular detection of ESBL and MBL genes using specific primers. Out of total 532 clinical samples 203 (38%) samples were identified as Pseudomonas aeruginosa. Out of positive samples 119 (58.6%) were confirmed MDR Pseudomonas aeruginosa. Out of 119 MDR positive samples, burn wounds showed the highest percentage 43 (36%), while least percentage 4 (3%) of MDR Pseudomonas aeruginosa was found in surgical wounds (P<0.05). All the selected isolates were resistant to β-lactams drugs and most effective drugs were tigecycline and colistin. Highest prevalence in the infected wound patients is blaNDM 14 (25.9%) producing P. aeruginosa and least blaKPC 1 (1.8%) producing P. aeruginosa. Results of the study concluded that surgical wounds showed the highest prevalence of MDR P. aeruginosa, suitable measures should be adopted to restrain this public health menace. en_US
dc.language.iso en en_US
dc.publisher Karachi:Pakistan Journal of Pharmaceutical Sciences, university of Karachi. en_US
dc.subject Wounds en_US
dc.subject Pseudomonas aeruginosa en_US
dc.subject multi drug resistance en_US
dc.subject antibiotic susceptibility profile en_US
dc.title Frequency of multi drug resistant Pseudomonas aeruginosa in different wound types of hospitalized patients en_US
dc.type Article en_US


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