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Chromatographic method development and validation for the determination of valsartan in biological fluid

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dc.contributor.author Ghayas, Sana
dc.contributor.author Shoaib, Muhammad Harris
dc.contributor.author Siddiqui, Fahad
dc.contributor.author Yousuf, Rabia Ismail
dc.contributor.author Masood, M. Ali
dc.contributor.author Anjum, Fakhsheena
dc.contributor.author Bushra, Rabia
dc.contributor.author Bashir, Lubna
dc.contributor.author Naz, Shazia
dc.contributor.author Muhammad, Iyad Naeem
dc.date.accessioned 2022-12-15T05:14:13Z
dc.date.available 2022-12-15T05:14:13Z
dc.date.issued 2017-11-05
dc.identifier.citation Ghayas, S., Shoaib, M. H., Siddiqui, F., Yousuf, R. I., Masood, M. A., Anjum, F., ... & Muhammad, I. N. (2017). Chromatographic method development and validation for the determination of valsartan in biological fluid. Pakistan Journal of Pharmaceutical Sciences, 30. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/15126
dc.description.abstract A swift, precise and simple HPLC bioanalytical technique with UV detection was established and validated for quantitative estimation of valsartan in human plasma. The analyte was separated from plasma by protein precipitation with acetonitrile and chromatographically separated on Zorbax SB-C18 (5µm, 4.6mm × 15cm) column. The solvent mixture system consisting of acetonitrile, water and glacial acetic acid (40:59:1 v/v), was pumped using isocratic mode at 1mL/min flow rate. Samples’ detection of drug was made spectrophotometrically at a wavelength of 264nm. The analyte response was instituted to be linear from 0.06 to 8µg/mL with a regression value of 0.999. The accuracy of the proposed method was ranged between 97.2-100.3% with 5% RSD. The analytical recovery (>95%) was consistently observed and satisfactory sample stability was also found at different environmental conditions. In conclusion the reported bio-analytical method is easy and robust that was successfully utilized in estimation of valsartan in a pharmacokinetic study. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject HPLC en_US
dc.subject plasma en_US
dc.subject valsartan en_US
dc.subject method validation en_US
dc.title Chromatographic method development and validation for the determination of valsartan in biological fluid en_US
dc.type Article en_US


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