PASTIC Dspace Repository

Effects of psoralen on chondrocyte degeneration in lumbar intervertebral disc of rats

Show simple item record

dc.contributor.author Yang, Libin
dc.contributor.author Sun, Xiaohui
dc.contributor.author Geng, Xiaolin
dc.date.accessioned 2022-12-15T06:11:43Z
dc.date.available 2022-12-15T06:11:43Z
dc.date.issued 2015-03-01
dc.identifier.citation Yang, L., Sun, X., & Geng, X. (2015). Effects of psoralen on chondrocyte degeneration in lumbar intervertebral disc of rats. Pakistan journal of pharmaceutical sciences, 28(supplement 2), S667-S670. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/15145
dc.description.abstract Discuss the internal mechanism of delaying degeneration of lumber intervertebral disc. The cartilage of lumbar intervertebral disc of SD rats was selected in vitro, then cultured by tissue explant method, and identified by HE staining, toluidine blue staining and immunofluorescence. The optimal concentration of psoralen was screened by cell proliferation assay and RT-PCR method. The cells in third generation with good growth situation is selected and placed in 6-well plate at concentration of 1×105 /well and its expression was tested. Compared to concentration of 0, the mRNA expression of Col2al (Collagen α) secreted by was up regulated chondrocyte of lumbar intervertebral disc at the concentration of 12.5 and 25µM (P<0.0 or P<0.01). The aggrecan mRNA of psoralen group was higher than blank control group (P<0.01); compared with IL-1β induced group, the mRNA expression of Col2al was significantly increased but the mRNA expression of ADAMTS-5 was significantly decreased in psoralen group (P<0.01). These findings suggest that, psoralen can remit the degeneration of lumbar intervertebral disc induced by IL-1β to some extent, and affect the related factors of IL-1β signaling pathway. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Psoralen en_US
dc.subject IL-1β en_US
dc.subject chondrocyte en_US
dc.subject intervertebral disc en_US
dc.subject degeneration en_US
dc.title Effects of psoralen on chondrocyte degeneration in lumbar intervertebral disc of rats en_US
dc.type Article en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account