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Analysis of genetics and chemical contents relation compared to commonly used Cissus quadrangularis L. and barcode markers of some Thailand Cissus species

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dc.contributor.author Sudmoon, Runglawan
dc.contributor.author Chaveerach, Arunrat
dc.contributor.author Tanee, Tawatchai
dc.date.accessioned 2022-12-15T06:17:31Z
dc.date.available 2022-12-15T06:17:31Z
dc.date.issued 2016-01-20
dc.identifier.citation Sudmoon, R., Chaveerach, A., & Tanee, T. (2016). Analysis of genetics and chemical contents relation compared to commonly used Cissus quadrangularis L. and barcode markers of some Thailand Cissus species. Pakistan Journal of Pharmaceutical Sciences, 29(1). en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/15155
dc.description.abstract Several Cissus species are commonly used as traditional and modified medicines, and their chemical constituents are major point for precise usage. However, C. quadrangularis is the only species for which the usages and the chemical composition have been reported. These data should be investigated for other species in the genus. Eight species namely C. assamica, C. carnosa, C. elongata, C. hastata, C. javana, C. pteroclada, C. quadrangular is and C. repens were evaluated for genetic relationships and chemical composition. Constructed dendrogram shows high-powered efficiency of inter-simple sequence repeat (ISSR) data used which can clearly identify different and identical species. Genetic similarity (S) value of the identical species is 0.86, whereas for different species the value can vary from 0.53 to 0.75. Four highly related species (S=0.64-0.72), C. assamica, C. carnosa, C. hastata and C. repens were selected to undergo chemical study by gas chromatography-mass spectrometry (GC-MS) on the methanol crude extract. Only one compound, β-sitosterol, found in the four species is identical to the compound reported from C. quadrangular is, where there were five identical chemicals found in the selected species. Species-specific barcode with rbcL region was constructed. Nucleotide variation was evaluated indicating genetic distance value of 0.025 to 0.072. en_US
dc.language.iso en en_US
dc.publisher Karachi:Pakistan Journal of Pharmaceutical Sciences, university of Karachi. en_US
dc.subject Cissus en_US
dc.subject inter-simple sequence repeat (ISSR) en_US
dc.subject medicinal plant en_US
dc.subject phytochemicals en_US
dc.subject DNA barcode en_US
dc.subject rbcL region en_US
dc.title Analysis of genetics and chemical contents relation compared to commonly used Cissus quadrangularis L. and barcode markers of some Thailand Cissus species en_US
dc.type Article en_US


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