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Effect of the serum of rats treated with Suo Quan pill on embryonic stem cells

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dc.contributor.author Xu, Jing- Jing
dc.contributor.author Huang, Ping
dc.contributor.author Xiang, Yun-Ya
dc.contributor.author Tan, Bo
dc.contributor.author Wu, Qing-He
dc.contributor.author Ze-Hui Qin, Hong-Ying Cao
dc.date.accessioned 2022-12-15T10:40:21Z
dc.date.available 2022-12-15T10:40:21Z
dc.date.issued 2018-01-04
dc.identifier.citation Xu, J. J., Huang, P., Xiang, Y. Y., Tan, B., Wu, Q. H., Qin, Z. H., & Cao, H. Y. (2018). Effect of the serum of rats treated with Suo Quan pill on embryonic stem cells. Pakistan Journal of Pharmaceutical Sciences, 31. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/15240
dc.description.abstract Suo Quan pill(SQP), a well-known and classical traditional Chinese medicine compound, consists of three traditional Chinese medicine: Alpinia oxyphylla Miq., Lindera aggregata (Sims) Kosterm., Dioscorea opposite. Its effect was summarized as supplementing kidney- yang and shrinkaging urination. This study evaluated the effects of the serum of rats treated with Suo Quan pill on embryonic stem cells(ES cells). Cell proliferation was detected by MTT assay. Cell cycle and apoptosis of ES cells were evaluated with flow cytometry. Nanog mRNA expression was verified by fluorescence quantitative PCR and Nanog protein in ES cells was determined by Western blot. The serum of SQP-treated rats not only promoted ES cells proliferation and Nanog expression in ES cells, but also inhibited H202 stimulated cell apoptosis. Furthermore, the serum of rats containing SQP affected the cell cycle distribution of ES cells, reducing the percentage of cells in G0/G1phase and increasing the percentage of cells in G2/M phase, increasing the proliferation index of ES cells. These results illustrate that the enhanced effect of SQP on ES cells proliferation is in part due to the increased expression of Nanog in ES cells, the accelerated cell cycle period and the inhibited apoptosis of ES cells. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Suo Quan pill en_US
dc.subject embryonic stem cells en_US
dc.subject serologic pharmacological method en_US
dc.subject Chinese medicine en_US
dc.title Effect of the serum of rats treated with Suo Quan pill on embryonic stem cells en_US
dc.type Article en_US


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