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CLONING, CHARACTERIZATION AND EXPRESSION OF ALLENE OXIDE CYCLASE GENE INVOLVED IN JASMONATE BIOSYNTHESIS FROM TORENIA FOURNIERI

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dc.contributor.author LIU, YAN
dc.contributor.author ZHAO, XIAO-MEI
dc.contributor.author HUANG, YAN
dc.contributor.author SUN, RU-YAN
dc.contributor.author BIN, JIN-HUA
dc.date.accessioned 2022-12-20T03:55:01Z
dc.date.available 2022-12-20T03:55:01Z
dc.date.issued 2012-06-20
dc.identifier.citation Liu, Y., Zhao, X. M., Huang, Y., Sun, R., & Bin, J. (2012). CLONING, CHARACTERIZATION AND EXPRESSION OF ALLENE OXIDE CYCLASE GENE INVOLVED IN JASMONATE BIOSYNTHESIS FROM TORENIA FOURNIERI. Pak. J. Bot, 44(3), 973-980. en_US
dc.identifier.issn 2070-3368
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/15319
dc.description.abstract Jasmonates are phytohormones that play important roles in plant defense and development. Allene oxide cyclase (AOC; EC 5.3.99.6) catalyzes a crucial step in the jasmonic acid biosynthetic pathway that forms the stereoisomeric precursor cis-(+)-12-oxophytodienoic acid. In the present study, a full-length cDNA of AOC was isolated from Torenia fournieri Lind., designated as TfAOC (GenBank accession no. JF417978). The cDNA was 954 bp in length. The 723 bp open reading frame encoded a protein of 240 amino acids with a putative chloroplast targeting sequence. The predicted molecular mass and the isoelectric point of the protein were 26.16 kDa and 8.60, respectively. Phylogenetic analysis reveals that TfAOC belongs to the AOC superfamily and is highly homologous to AOC proteins from other plant species. RT-PCR analysis revealed that TfAOC mRNA was expressed in all organs of plants, with highest expression in leaves and lowest in roots. High expression level was also detected in pistils and anthers. The level of methyl jasmonate was correlated with the expression of TfAOC mRNA. These results suggest that JA is involved in the pistil and anther development in T. fournieri. en_US
dc.language.iso en en_US
dc.publisher Karachi: Pakistan Botanical Society en_US
dc.title CLONING, CHARACTERIZATION AND EXPRESSION OF ALLENE OXIDE CYCLASE GENE INVOLVED IN JASMONATE BIOSYNTHESIS FROM TORENIA FOURNIERI en_US
dc.type Article en_US


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