dc.contributor.author |
JINJUAN SHEN |
|
dc.contributor.author |
PINGZHONG CAI |
|
dc.contributor.author |
FENG QING |
|
dc.contributor.author |
ZHIYONG ZHANG |
|
dc.contributor.author |
GUIXUE WANG |
|
dc.date.accessioned |
2022-12-20T04:49:59Z |
|
dc.date.available |
2022-12-20T04:49:59Z |
|
dc.date.issued |
2012-04-20 |
|
dc.identifier.citation |
Shen, J., Cai, P., Qing, F., Zhang, Z., & Wang, G. (2012). A primary study of high performance transgenic rice through maize Ubi-1 promoter fusing selective maker gene. Pakistan Journal of Botany, 44(2), 501-506. |
en_US |
dc.identifier.issn |
2070-3368 |
|
dc.identifier.uri |
http://142.54.178.187:9060/xmlui/handle/123456789/15354 |
|
dc.description.abstract |
Based on the expression vector pBI121, we successfully constructed a plant overexpression vector of Hspa4 gene
fusing with selective maker gene (hygromycin-resistance gene) driven by the Ubi-1 promoter (pBI121-Ubi-Hpt-Hspa4,
p121UHH). The plant expression vectors p121UHH and pCAMBIA1301-Ubi-Hspa4 (p1301UH) were transformed into the
rice callus, mediated by Agrobacterium tumefaciens. We screened 17 p121UHH-positive transgenic plants and 15
p1301UH-positive transgenic plants by the hygromycin-resistance gene. The pick-up rate of the resistance callus was 51.7%
and 42.5%, respectively, and the rate of regeneration for the resistance callus was 51.2% and 49.1%, respectively. The result
of polymerase chain reaction (PCR) identification indicated that the pick-up rate of positive transgenic plants was 51.7% and
42.5% and the total transformation efficiency was 16.5% and 6.2%, and the former was 2.66 times of the later. The results
of the experiment indicate that the possibility of the appearance of false positive results in the fusing of a plant overexpression vector with a selective maker gene is much less |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Karachi: Pakistan Botanical Society, University of Karachi |
en_US |
dc.title |
A PRIMARY STUDY OF HIGH PERFORMANCE TRANSGENIC RICE THROUGH MAIZE UBI-1 PROMOTER FUSING SELECTIVE MAKER GENE |
en_US |
dc.type |
Article |
en_US |