Abstract:
The chloroplast gene matK, located in the intron of chloroplast trnK, encodes maturase, and variations of matK provide
substantial resolution for phylogenetic analyses at intergeneric levels. Sequence data from 127 species (including subspecies
and varieties) of Brassicaceae and one outgroup specie (Cleome gynandra) were used to construct the phylogeny of this
family and elucidate the phylogenetic relationships therein using the neighbor-joining, maximum parsimony, and maximum
likelihood methods. The phylogenetic results generally confirmed recently established tribal alignments and indicated that
most of the 27 tribes were assigned to Lineages I–III. We found that the Orychophragmus violaceus complex, including O.
violaceus, O. taibaiensis, O. hupehensis, and O. diffuses, which are native to China, should be subsumed under Lineage II,
and was most closely associated with the tribe Brassiceae. Arabis was confirmed to be polyphyletic and one subclade shared
a sister relationship with Boechereae, while A. alpine related species formed the other clade, which was not associated with
any tribes. Previous analyses placed Conringia planisiliqua in tribe Brassiceae, but it was included within Isatideae in the
current analyses, supporting previous hypotheses that it was a member of this tribe.
