Abstract:
A comparative study on three clonal propagation systems was carried out on banana cv. Pisang Awak for production of
plantlets. The temporary immersion system (TIS) was found to be the most suitable method for in vitro propagation of
Pisang Awak plantlets as compared to the gelled medium and the shake flask system. There was high shoot proliferation and
more roots formation when they were cultured in MS medium supplemented with 5.0 mg/L BA in TIS. The plantlets
produced from gelled medium system and TIS also acclimatized well in greenhouse except those derived from the shake
flask system where none of the plantlet survived after transferred to the soil. The optimized TIS propagation protocol was
found to be effective for mass propagation of other three banana cultivars, Pisang Raja, Pisang Rastali and Pisang Berangan.
