dc.contributor.author |
BALOCH, MUHAMMAD JURIAL |
|
dc.contributor.author |
JIM DUNWELL |
|
dc.contributor.author |
KHAN, NAQIB ULLAH |
|
dc.contributor.author |
KHAKWANI, ABDUL AZIZ |
|
dc.contributor.author |
MIKE DENNET |
|
dc.contributor.author |
JATOI, WAJID ALI |
|
dc.date.accessioned |
2022-12-21T04:11:09Z |
|
dc.date.available |
2022-12-21T04:11:09Z |
|
dc.date.issued |
2012-04-20 |
|
dc.identifier.citation |
Baloch, M. J., Dunwell, J., Khan, N. U., Khakwani, A. A., Dennett, M., & Jatoi, W. A. (2012). Profiling dehydrin gene sequence and physiological parameters in drought tolerant and susceptible spring wheat cultivars. Pakistan Journal of Botany, 44(2), 801-806. |
en_US |
dc.identifier.issn |
2070-3368 |
|
dc.identifier.uri |
http://142.54.178.187:9060/xmlui/handle/123456789/15400 |
|
dc.description.abstract |
Physiological and yield traits such as stomatal conductance (mmol m-2
s-1
), Leaf relative water content (RWC %) and
grain yield per plant were studied in a separate experiment. Results revealed that five out of sixteen cultivars viz. Anmol,
Moomal, Sarsabz, Bhitai and Pavan, appeared to be relatively more drought tolerant. Based on morphophysiological results,
studies were continued to look at these cultivars for drought tolerance at molecular level. Initially, four well recognized
primers for dehydrin genes (DHNs) responsible for drought induction in T. durum L., T. aestivum L. and O. sativa L. were
used for profiling gene sequence of sixteen wheat cultivars. The primers amplified the DHN genes variably like Primer
WDHN13 (T. aestivum L.) amplified the DHN gene in only seven cultivars whereas primer TdDHN15 (T. durum L.)
amplified all the sixteen cultivars with even different DNA banding patterns some showing second weaker DNA bands.
Third primer TdDHN16 (T. durum L.) has shown entirely different PCR amplification prototype, specially showing two
strong DNA bands while fourth primer RAB16C (O. sativa L.) failed to amplify DHN gene in any of the cultivars.
Examination of DNA sequences revealed several interesting features. First, it identified the two exon/one intron structure of
this gene (complete sequences were not shown), a feature not previously described in the two database cDNA sequences
available from T. aestivum L. (gi|21850). Secondly, the analysis identified several single nucleotide polymorphisms (SNPs),
positions in gene sequence. Although complete gene sequence was not obtained for all the cultivars, yet there were a total of
38 variable positions in exonic (coding region) sequence, from a total gene length of 453 nucleotides. Matrix of SNP shows
these 37 positions with individual sequence at positions given for each of the 14 cultivars (sequence of two cultivars was not
obtained) included in this analysis. It demonstrated a considerable diversity for this gene with only three cultivars i.e. TJ-83,
Marvi and TD-1 being similar to the consensus sequence. All other cultivars showed a unique combination of SNPs. In
order to prove a functional link between these polymorphisms and drought tolerance in wheat, it would be necessary to
conduct a more detailed study involving directed mutation of this gene and DHN gene expression. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Karachi: Pakistan Botanical Society, University of Karachi |
en_US |
dc.title |
PROFILING DEHYDRIN GENE SEQUENCE AND PHYSIOLOGICAL PARAMETERS IN DROUGHT TOLERANT AND SUSCEPTIBLE SPRING WHEAT CULTIVARS |
en_US |
dc.type |
Article |
en_US |