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PROFILING DEHYDRIN GENE SEQUENCE AND PHYSIOLOGICAL PARAMETERS IN DROUGHT TOLERANT AND SUSCEPTIBLE SPRING WHEAT CULTIVARS

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dc.contributor.author BALOCH, MUHAMMAD JURIAL
dc.contributor.author JIM DUNWELL
dc.contributor.author KHAN, NAQIB ULLAH
dc.contributor.author KHAKWANI, ABDUL AZIZ
dc.contributor.author MIKE DENNET
dc.contributor.author JATOI, WAJID ALI
dc.date.accessioned 2022-12-21T04:11:09Z
dc.date.available 2022-12-21T04:11:09Z
dc.date.issued 2012-04-20
dc.identifier.citation Baloch, M. J., Dunwell, J., Khan, N. U., Khakwani, A. A., Dennett, M., & Jatoi, W. A. (2012). Profiling dehydrin gene sequence and physiological parameters in drought tolerant and susceptible spring wheat cultivars. Pakistan Journal of Botany, 44(2), 801-806. en_US
dc.identifier.issn 2070-3368
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/15400
dc.description.abstract Physiological and yield traits such as stomatal conductance (mmol m-2 s-1 ), Leaf relative water content (RWC %) and grain yield per plant were studied in a separate experiment. Results revealed that five out of sixteen cultivars viz. Anmol, Moomal, Sarsabz, Bhitai and Pavan, appeared to be relatively more drought tolerant. Based on morphophysiological results, studies were continued to look at these cultivars for drought tolerance at molecular level. Initially, four well recognized primers for dehydrin genes (DHNs) responsible for drought induction in T. durum L., T. aestivum L. and O. sativa L. were used for profiling gene sequence of sixteen wheat cultivars. The primers amplified the DHN genes variably like Primer WDHN13 (T. aestivum L.) amplified the DHN gene in only seven cultivars whereas primer TdDHN15 (T. durum L.) amplified all the sixteen cultivars with even different DNA banding patterns some showing second weaker DNA bands. Third primer TdDHN16 (T. durum L.) has shown entirely different PCR amplification prototype, specially showing two strong DNA bands while fourth primer RAB16C (O. sativa L.) failed to amplify DHN gene in any of the cultivars. Examination of DNA sequences revealed several interesting features. First, it identified the two exon/one intron structure of this gene (complete sequences were not shown), a feature not previously described in the two database cDNA sequences available from T. aestivum L. (gi|21850). Secondly, the analysis identified several single nucleotide polymorphisms (SNPs), positions in gene sequence. Although complete gene sequence was not obtained for all the cultivars, yet there were a total of 38 variable positions in exonic (coding region) sequence, from a total gene length of 453 nucleotides. Matrix of SNP shows these 37 positions with individual sequence at positions given for each of the 14 cultivars (sequence of two cultivars was not obtained) included in this analysis. It demonstrated a considerable diversity for this gene with only three cultivars i.e. TJ-83, Marvi and TD-1 being similar to the consensus sequence. All other cultivars showed a unique combination of SNPs. In order to prove a functional link between these polymorphisms and drought tolerance in wheat, it would be necessary to conduct a more detailed study involving directed mutation of this gene and DHN gene expression. en_US
dc.language.iso en en_US
dc.publisher Karachi: Pakistan Botanical Society, University of Karachi en_US
dc.title PROFILING DEHYDRIN GENE SEQUENCE AND PHYSIOLOGICAL PARAMETERS IN DROUGHT TOLERANT AND SUSCEPTIBLE SPRING WHEAT CULTIVARS en_US
dc.type Article en_US


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