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AGROBACTERIUM MEDIATED TRANSFORMATION OF BRASSICA JUNCEA (L.) CZERN. WITH CHITINASE GENE CONFERRING RESISTANCE AGAINST FUNGAL INFECTIONS

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dc.contributor.author BASHIR AHMAD
dc.contributor.author AMBREEN
dc.contributor.author KHAN, MUHAMMAD SAYYAR
dc.contributor.author ALI HAIDER
dc.contributor.author KHAN, IBRAR
dc.date.accessioned 2022-12-26T06:43:28Z
dc.date.available 2022-12-26T06:43:28Z
dc.date.issued 2015-03-01
dc.identifier.citation Ahmad, B., Ambreen, M. S. K. A. H., & Khan, I. B. R. A. R. (2015). Agrobacterium mediated transformation of Brassica juncea (L.) Czern. with chitinase gene conferring resistance against fungal infections. Pak. J. Bot, 47(1), 211-216. en_US
dc.identifier.issn 2070-3368
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/15445
dc.description.abstract Brassica juncea (Czern & Coss., L.) is an important oilseed crop. Since it is attacked by several bacterial and fungal diseases, therefore, we developed an easy and simple protocol for the regeneration and transformation of B. juncea variety RAYA ANMOL to give rise to transgenic plants conferring resistance against various fungal diseases. The transformation was carried out using Agrobacterium with Chitinase gene. This gene was isolated from Streptomyces griseus HUT6037. We used two types of explants for transformation i.e. hypocotyls and cotyledons. Only hypocotyls explants showed good results regarding callus initiation. Different hormonal concentrations were applied i.e. BAP 2, 4 and 6 mgL-1 and NAA 0.1, 0.2 and 0.3 mgL-1. However, high transformation efficiency was observed by supplementing the medium with combination of 2 mgL-1 BAP and 0.2 mgL-1 for initiation of callus. Similarly 10 mgL-1 kanamycin and 200 mgL-1 cefotaxime also proved successful for the selection of transformed callus. In order to confirm the presence of transgenic callus Polymerase chain reaction was performed using specific primers for Chitinase gene. en_US
dc.language.iso en en_US
dc.publisher Karachi: Pakistan Botanical Society en_US
dc.subject Agrobacterium en_US
dc.subject Brassica juncea en_US
dc.subject Chitinase gene en_US
dc.subject Fungal infections en_US
dc.title AGROBACTERIUM MEDIATED TRANSFORMATION OF BRASSICA JUNCEA (L.) CZERN. WITH CHITINASE GENE CONFERRING RESISTANCE AGAINST FUNGAL INFECTIONS en_US
dc.type Article en_US


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