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EMBRYOGENIC CALLUS INDUCTION, SOMATIC EMBRYOGENESIS, REGENERATION AND HISTOLOGICAL STUDIES OF KINNOW MANDARIN (CITRUS RETICULATA BLANCO L.) FROM NUCELLAR EMBRYO AND EPICOTYL REGION

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dc.contributor.author KAZMI, SYEDA KAHKASHAN
dc.contributor.author KHAN, SAIFULLAH
dc.contributor.author NURUL KABIR
dc.contributor.author AMEER AHMED MIRBAHAR
dc.contributor.author MARIAM RAZIQ
dc.contributor.author NAHEED KAUSER
dc.date.accessioned 2022-12-27T05:42:03Z
dc.date.available 2022-12-27T05:42:03Z
dc.date.issued 2015-03-15
dc.identifier.citation Kazmi, S. K., Khan, S., Kabir, N. U. R. U. L., Mirbahar, A. A., Raziq, M., & Kauser, N. A. H. E. E. D. (2015). Embryogenic callus induction, somatic embryogenesis, regeneration and histological studies of kinnow mandarin (Citrus reticulata blanco L.) From Nucellar Embryo and Epicotyl Region. Pak. J. Bot, 47(1), 305-310. en_US
dc.identifier.issn 2070-3368
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/15459
dc.description.abstract Citrus is one of the most dominant horticultural fruit crop in the world. The objective of this study was to develop an efficient protocol for invitro embryogenic callus induction and regeneration of Kinnow mandarin. In this study, different concentrations of 2,4-D (0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 mg/L) along with constant 0.5mg/L BAP were used to check the effective response of epicotyl segments and nucellar tissues on callus induction and regeneration. It was observed that MS medium containing 2.5 mg/L 2,4-D and 0.5mg/L BAP supplemented with 0.5gm/L malt extract was suitable for embryogenic callus induction from both epicotyl segments and nucellar embryonic tissues. Callus mbryogenesis was achieved on simple MS medium where as regeneration was obtained on MT medium containing 0.5mg/L BAP and 0.5mg/L Kinetin along with 0.5gm/L malt extract. It was observed that the nucellar embryos are the best explants for efficient callus induction and regeneration. In order to ensure the efficient regeneration ability of nucellar callus histological studies were performed, which showed that nucellar embryos and its calli have more chloroplasts as compared to epicotyl segments, which enhanced regeneration ability of nucellar embryos. en_US
dc.language.iso en en_US
dc.publisher Karachi: Pakistan Botanical Society en_US
dc.subject Indirect regeneration en_US
dc.subject Nucellar embryos en_US
dc.subject Toluidine Blue O staining en_US
dc.title EMBRYOGENIC CALLUS INDUCTION, SOMATIC EMBRYOGENESIS, REGENERATION AND HISTOLOGICAL STUDIES OF KINNOW MANDARIN (CITRUS RETICULATA BLANCO L.) FROM NUCELLAR EMBRYO AND EPICOTYL REGION en_US
dc.type Article en_US


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