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MICROPROPAGATION OF EUCALYPTUS SALIGNA SM. FROM COTYLEDONARY NODES

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dc.contributor.author DA SILVA, ANDRÉ LUÍS LOPES
dc.contributor.author GOLLO, ANDRÉ LUIZ
dc.contributor.author BRONDANI, GILVANO EBLING
dc.contributor.author HORBACH, MICHELI ANGÉLICA
dc.contributor.author DE OLIVEIRA, LEANDRO SILVA
dc.contributor.author MACHADO, MARÍLIA PEREIRA
dc.contributor.author DE LIMA, KHIOMARA KHÉMELI DELLANI
dc.contributor.author COSTA, JEFFERSON DA LUZ
dc.date.accessioned 2022-12-27T05:44:33Z
dc.date.available 2022-12-27T05:44:33Z
dc.date.issued 2015-03-16
dc.identifier.citation Da Silva, A. L. L., Gollo, A. L., Brondani, G. E., Horbach, M. A., Oliveira, L. S., Machado, M. P., ... & Costa, J. (2015). Micropropagation of Eucalyptus saligna Sm. from cotyledonary nodes. Pak. J. Bot, 47(1), 311-318. en_US
dc.identifier.issn 2070-3368
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/15460
dc.description.abstract Eucalyptus saligna is an important woody plant used to lumber and cellulose. The aim of this research was to establish a protocol for micropropagation of this species from cotyledonary nodes. Plantlets with 16 days old were used as a donor explants. The induction of cotyledonary nodes consisted of two parts: a dark culture followed by a light culture. Basal medium was MS added with 30g.L-1 sucrose, 10% coconut water and solidified with 7g.L-1 agar. For the dark culture the media were supplemented with 3.6μM NAA (Naftalenoacetic acid) and 4.4 μM BAP (6-Benzylaminopurine) and for the light culture the media were supplemented with 2.7μM NAA and 1.1 μM BAP. The period for dark and light culture was 20 days. Shoots were multiplied on MS medium, 30 g.L-1 sucrose supplemented with 1.1 μM BAP. Shoots were elongated on MS medium free of plant growth regulators. Shoots were rooting on half-strength MS salts. Acclimatization was performed in a hydroponics floating system. Moreover, the shoot multiplication in liquid medium with different CaCl2 levels was carried out under agitation. Organogenesis of cotyledonary nodes was characterized by simultaneous occurrence of shoot and callus. Shoots presented hyperhydricity under liquid medium, however, the CaCl2 reduces the hyperhydricity in liquid medium; nevertheless, it had been not effective in eliminating hyperhydricity due to toxicity of chlorine. The hydroponics acclimatization results in 90% plant survival. An efficient protocol for micropropagation of E. saligna was suitable established and can be used for clonal propagation or genetic transformation. en_US
dc.language.iso en en_US
dc.publisher Karachi: Pakistan Botanical Society en_US
dc.subject Hyperhydricity en_US
dc.subject Nitrogen source en_US
dc.subject Eucalypt en_US
dc.subject Phenolic oxidation en_US
dc.subject Browning explant en_US
dc.subject Callogenesis en_US
dc.title MICROPROPAGATION OF EUCALYPTUS SALIGNA SM. FROM COTYLEDONARY NODES en_US
dc.type Article en_US


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