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COMPARATIVE STUDY ON THE NOVEL AMYLASES PURIFIED FROM APPLE AND ORANGE SEEDS

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dc.contributor.author MIRZA, UMAIRA SAEED
dc.contributor.author MUHAMMAD ANJUM ZIA
dc.contributor.author SAMREEN RASUL
dc.contributor.author TEHREEMA IFTIKHAR
dc.date.accessioned 2023-01-05T07:34:10Z
dc.date.available 2023-01-05T07:34:10Z
dc.date.issued 2014-01-03
dc.identifier.citation Mirza, U. S., Zia, M. A., Rasul, S., & Iftikhar, T. (2013). Comparative study on the novel amylases purified from apple and orange seeds. Pakistan Journal of Botany, 45(6), 2075-2078. en_US
dc.identifier.issn 2070-3368
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/15669
dc.description.abstract Amylases are starch-digesting enzymes that are naturally present in seeds, fruits, tubers, roots, stem and pith of all plants. The present study is focused on the comparative studies on the α-amylase from apple and orange seeds to seek the better source. The enzyme extract was subjected to 40-60% salt precipitation. Further purification was carried out by traditional chromatographic procedures. The crude extracts obtained from apple and orange seeds presented the activities of 4.43 and 3.48 U mL-1 respectively. The purification through ion exchange and size exclusion chromatography resulted in an increase in specific activity for the apple source. The fold purification for the enzyme was 257.78 and 11.17 from apple and orange seeds, respectively. The study concluded that apple seeds are better source for purified α-amylase as compared to orange seeds. This work will also help to explore the natural sources like seeds for the isolation and purification of enzymes. en_US
dc.language.iso en en_US
dc.publisher Karachi: Pakistan Botanical Society en_US
dc.title COMPARATIVE STUDY ON THE NOVEL AMYLASES PURIFIED FROM APPLE AND ORANGE SEEDS en_US
dc.type Article en_US


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