dc.contributor.author |
SAIQA ILYAS |
|
dc.contributor.author |
SHAGUFTA NAZ |
|
dc.contributor.author |
FARAH ASLAM |
|
dc.contributor.author |
ZAHIDA PARVEEN |
|
dc.contributor.author |
AAMIR ALI |
|
dc.date.accessioned |
2023-01-05T09:07:59Z |
|
dc.date.available |
2023-01-05T09:07:59Z |
|
dc.date.issued |
2014-05-07 |
|
dc.identifier.citation |
Ilyas, S., Naz, S., Aslam, F., Parveen, Z., & Ali, A. (2014). Chemical composition of essential oil from in vitro grown Peperomia obtusifolia through GC-MS. Pak. J. Bot, 46(2), 667-672. |
en_US |
dc.identifier.issn |
2070-3368 |
|
dc.identifier.uri |
http://142.54.178.187:9060/xmlui/handle/123456789/15721 |
|
dc.description.abstract |
Apical meristems and nodal plant parts were used for mass propagation of Peperomia obtusifolia. Different
concentrations of BAP (6-benzylaminopurine), TDZ (Thidiazuron) and KIN (Kinetin) were used in MS medium. The highest shoot proliferation and multiplication formation occurred in the MS basal medium containing 1.0 mg/mL BAP. Maximum number of shoots and shoot lengths were 9.80 ±0.50 and 35.40 ± 2.92 cm respectively. Rooting response was the best in MS basal medium fortified with1.0 mg/mL BAP plus 0.5 mg/mL NAA (α-naphthalene acetic acid). For acclimatization, the rooted plantlets were transferred to the greenhouse. The volatile oil of this In vitro grown P. obtusifolia was extracted by hydro-distillation and investigated by gas chromatography/mass spectrometry (GC–MS). The chromatographic analysis of oil showed 35 constituents of which, 16 volatile compounds contributing 65.0% of the total oil constituents could be identified. The major components identified in this oil were sesquiterpenes such as caryophyllene (17.17%), apiol (16.65%), α-cardinol (2.12%) and α-caryophyllene (1.90%). The monoterpenes such as R-α-Pinene (1.00%), camphene (0.84%), borneal (0.32%) and limonene (0.25%) were also identified. The tau.-muurolol (0.68%), τElemene (0.63%), copaene (0.43%) and tau.-cadinol (0.27%) were present comparatively in minor percentages. The
caryophyllene oxide (2.95%) was oxygenated sesquiterpene, 3,5-Dimethoxy-4-hydroxycinnamic acid (0.53%) and asarone (0.41%) were phenyl-propanoids. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Karachi: Pakistan Botanical Society |
en_US |
dc.title |
CHEMICAL COMPOSITION OF ESSENTIAL OIL FROM IN VITRO GROWN PEPEROMIA OBTUSIFOLIA THROUGH GC-MS |
en_US |
dc.type |
Article |
en_US |