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PROCESS OPTIMIZATION BY RESPONSE SURFACE METHODOLOGY FOR EXTRACELLULAR ALKALINE PROTEASE PRODUCTION FROM BACILLUS SUBTILIS

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dc.contributor.author ZAREENA MUSHTAQ
dc.contributor.author AHMAD ADNAN
dc.contributor.author ZAHID MEHMOOD
dc.contributor.author QURATULAIN SYED
dc.date.accessioned 2023-01-05T09:10:43Z
dc.date.available 2023-01-05T09:10:43Z
dc.date.issued 2014-05-12
dc.identifier.citation Mushtaq, Z., Adnan, A., Mehmood, Z., & SYED, Q. (2014). Process optimization by response surface methodology for extracellular alkaline protease production from Bacillus subtilis. Pak J Bot, 46(2), 699-704. en_US
dc.identifier.issn 2070-3368
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/15726
dc.description.abstract Three microbial cultures Bacillus subtilis DSM 1970, Bacillus subtilis GCU-8 and Bacillus licheniformis DSM 1969 were screened for protease production by casein agar plate method. Among these Bacillus subtilis GCU-8 was found to be the most potent protease producer in wide pH range (5.0 to 8.0). Fermentation conditions were optimized for the production of alkaline protease using two statistical tools: Placket Burmen Model for linear regression study and Response Surface Model for interactive effects of significant factors on production. The alkaline protease was optimally produced after 48 hours of incubation at 37o C in fermentation media containing equal amounts of substrates (soybean meal and wheat bran,7.5 g), MgSO4.7H2O, 0.10 g and yeast extract 0.55 g. The protease was purified to homogeneity by salt precipitation, ionexchange chromatography and size exclusion chromatography. The homogeneity and molecular weights were checked by SDS-PAGE. The protease was 45 KDa protein, predominantly alkaline and optimally active at pH 8.0. en_US
dc.language.iso en en_US
dc.publisher Karachi: Pakistan Botanical Society en_US
dc.title PROCESS OPTIMIZATION BY RESPONSE SURFACE METHODOLOGY FOR EXTRACELLULAR ALKALINE PROTEASE PRODUCTION FROM BACILLUS SUBTILIS en_US
dc.type Article en_US


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