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OPTIMIZATION OF AGROBACTERIUM TUMEFACIENS MEDIATED TRANSFORMATION IN EUCALYPTUS CAMALDULENSIS

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dc.contributor.author AMMARA AHAD
dc.contributor.author ASMA MAQBOOL
dc.contributor.author MALIK, KAUSER ABDULLA
dc.date.accessioned 2023-01-05T09:13:01Z
dc.date.available 2023-01-05T09:13:01Z
dc.date.issued 2014-05-17
dc.identifier.citation Ahad, A., Maqbool, A., & Malik, K. A. (2014). Optimization of Agrobacterium tumefaciens mediated transformation in Eucalyptus camaldulensis. Pak J Bot, 76(2), 735-774. en_US
dc.identifier.issn 2070-3368
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/15730
dc.description.abstract This study was conducted to optimize Agrobacterium tumefaciens mediated transformation for Eucalyptus camaldulensis. Transformation was done by using LBA4404 containing binary plasmid pGA482 with uidA (Gus) gene under CamV35S promoter and nptII gene under nos promoter. For optimization, different explants (Cotyledonary leaves, plantlet leaves and hypocotyls of young In vitro plants and calli) with and without preculture were infected with a range of optical densities (O.D.600nm=0.3-0.6). Effect of different concentrations of Acetosyringone, infection time and co-cultivation time on transformation efficiency was evaluated. Confirmation of transformation was done through GUS histochemical staining & through PCR. Callogenesis and regeneration was found fast on MS medium containing 0.5 mg/L NAA and 1.5 mg/L BAP. Highest transformation efficiency was obtained with bacterial suspension of O.D.600nm = 0.5 for non- precultured explants and O.D.600nm=0.3 for precultured explants. en_US
dc.language.iso en en_US
dc.publisher Karachi: Pakistan Botanical Society en_US
dc.title OPTIMIZATION OF AGROBACTERIUM TUMEFACIENS MEDIATED TRANSFORMATION IN EUCALYPTUS CAMALDULENSIS en_US
dc.type Article en_US


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