dc.contributor.author |
WANG, XUEZHENG |
|
dc.contributor.author |
SHANG, LIMIN |
|
dc.contributor.author |
LUAN, FEISHI |
|
dc.date.accessioned |
2023-01-05T09:24:36Z |
|
dc.date.available |
2023-01-05T09:24:36Z |
|
dc.date.issued |
2013-02-19 |
|
dc.identifier.citation |
Wang, X., Shang, L., & Luan, F. (2013). A highly efficient regeneration system for watermelon (Citrullus lanatus Thunb.). Pak. J. Bot, 45(1), 145-150. |
en_US |
dc.identifier.issn |
2070-3368 |
|
dc.identifier.uri |
http://142.54.178.187:9060/xmlui/handle/123456789/15751 |
|
dc.description.abstract |
We developed a high frequency watermelon regeneration system using two inbred lines of watermelon (Citrullus lanatus), ‘W1-4’ and ‘W1-12’. Shoots were induced from cotyledonary nodes cultured on Murashige and Skoog (MS) basal medium solidified with agar (7.0 g/L) and containing various concentrations of cytokinin (6-benzyladenine; 6-BA) and auxin (indoleacetic acid; IAA). The highest rate of bud organogenesis was on MS medium containing 1.5 mg/L 6-BA + 0.2 mg/L IAA for ‘W1-4’ and on MS medium containing 1.0 mg/L 6-BA + 0.1 mg/L IAA for ‘W1-12’. The regeneration rate was higher in ‘W1-12’ than in ‘W1-4’. The best medium for shoot elongation in both inbred lines was MS containing 0.05 mg/L 6-BA. Regenerated plants showed the best rates of root formation on 1/2 MS containing 0.1 mg/L IAA. The rooted plants were carefully washed to remove all medium from the roots, and then transferred to soil in a greenhouse. The plants showed a 100% survival rate when transferred to soil. This highly efficient regeneration system will be useful for regenerating plants in genetic engineering applications, and is a useful tool for further genetic transformation studies on watermelon. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Karachi: Pakistan Botanical Society |
en_US |
dc.title |
A HIGHLY EFFICIENT REGENERATION SYSTEM FOR WATERMELON (CITRULLUS LANATUS THUNB.) |
en_US |
dc.type |
Article |
en_US |