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A HIGHLY EFFICIENT REGENERATION SYSTEM FOR WATERMELON (CITRULLUS LANATUS THUNB.)

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dc.contributor.author WANG, XUEZHENG
dc.contributor.author SHANG, LIMIN
dc.contributor.author LUAN, FEISHI
dc.date.accessioned 2023-01-05T09:24:36Z
dc.date.available 2023-01-05T09:24:36Z
dc.date.issued 2013-02-19
dc.identifier.citation Wang, X., Shang, L., & Luan, F. (2013). A highly efficient regeneration system for watermelon (Citrullus lanatus Thunb.). Pak. J. Bot, 45(1), 145-150. en_US
dc.identifier.issn 2070-3368
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/15751
dc.description.abstract We developed a high frequency watermelon regeneration system using two inbred lines of watermelon (Citrullus lanatus), ‘W1-4’ and ‘W1-12’. Shoots were induced from cotyledonary nodes cultured on Murashige and Skoog (MS) basal medium solidified with agar (7.0 g/L) and containing various concentrations of cytokinin (6-benzyladenine; 6-BA) and auxin (indoleacetic acid; IAA). The highest rate of bud organogenesis was on MS medium containing 1.5 mg/L 6-BA + 0.2 mg/L IAA for ‘W1-4’ and on MS medium containing 1.0 mg/L 6-BA + 0.1 mg/L IAA for ‘W1-12’. The regeneration rate was higher in ‘W1-12’ than in ‘W1-4’. The best medium for shoot elongation in both inbred lines was MS containing 0.05 mg/L 6-BA. Regenerated plants showed the best rates of root formation on 1/2 MS containing 0.1 mg/L IAA. The rooted plants were carefully washed to remove all medium from the roots, and then transferred to soil in a greenhouse. The plants showed a 100% survival rate when transferred to soil. This highly efficient regeneration system will be useful for regenerating plants in genetic engineering applications, and is a useful tool for further genetic transformation studies on watermelon. en_US
dc.language.iso en en_US
dc.publisher Karachi: Pakistan Botanical Society en_US
dc.title A HIGHLY EFFICIENT REGENERATION SYSTEM FOR WATERMELON (CITRULLUS LANATUS THUNB.) en_US
dc.type Article en_US


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