TRANSFER OF THE GAFP AND NPI, TWO DISEASE-RESISTANT GENES, INTO A PHALAENOPSIS BY AGROBACTERIUM TUMEFACIENS

LI, JIE; KUANG, PING; LIU, REN-DAO; WANG, DAN; WANG, ZHI-NA; HUANG, MIN-REN

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dc.contributor.author	LI, JIE
dc.contributor.author	KUANG, PING
dc.contributor.author	LIU, REN-DAO
dc.contributor.author	WANG, DAN
dc.contributor.author	WANG, ZHI-NA
dc.contributor.author	HUANG, MIN-REN
dc.date.accessioned	2023-01-10T04:12:33Z
dc.date.available	2023-01-10T04:12:33Z
dc.date.issued	2013-11-13
dc.identifier.citation	Li, J., Kuang, P., Liu, R. D., Wang, D., Wang, Z. N., & Huang, M. R. (2013). Transfer of the gafp and npi, two disease-resistant genes, into a phalaenopsis by Agrobacterium tumefaciens. Pakistan Journal of Botany, 45(5), 1761-1766.	en_US
dc.identifier.issn	2070-3368
dc.identifier.uri	http://142.54.178.187:9060/xmlui/handle/123456789/15827
dc.description.abstract	Gastrodia Antifungal Protein (GAFP) and Neutrophils Peptide-I (NPI), two disease-resistant genes were successfully transferred into the Dtps.Tailin Angel Phalaenopsis by using Agrobacterium strain off gradual methods. Two hundred and eighty kanamycin-resistant plants were regenerated by this method. PCR, Southern blot and RT-PCR confirmed that GAFP and NPI genes have been integrated into the genome of Phalaenopsis. In vitro antibiosis assay of the Colletotrichum gloeosporioides Sacc suggested that the transgenic plants were disease-resistant. Disease-resistance experiments proved that both GAFP and NPI genes were expressed efficiently in Phalaenopsis.	en_US
dc.language.iso	en	en_US
dc.publisher	Karachi: Pakistan Botanical Society	en_US
dc.title	TRANSFER OF THE GAFP AND NPI, TWO DISEASE-RESISTANT GENES, INTO A PHALAENOPSIS BY AGROBACTERIUM TUMEFACIENS	en_US
dc.type	Article	en_US