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Extraction, formulation and characterization of an in vitro and ex-vivo evaluation of Thymus serpyllum L. (Thymus oil) from topical preparations using dialysis cellulose membrane and natural rabbit skin

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dc.contributor.author Rahman Gul
dc.contributor.author Syed Umer Jan
dc.contributor.author Mahmood Ahmed
dc.contributor.author Syed Faridullah
dc.contributor.author Muhammad Akhtar
dc.date.accessioned 2023-01-13T07:17:26Z
dc.date.available 2023-01-13T07:17:26Z
dc.date.issued 2019-07-14
dc.identifier.citation Gul, R., Jan, S. U., Ahmed, M., Faridullah, S., & Akhtar, M. (2019). Extraction, formulation and characterization of an in vitro and ex-vivo evaluation of Thymus serpyllum L.(Thymus oil) from topical preparations using dialysis cellulose membrane and natural rabbit skin. Pakistan Journal of Pharmaceutical Sciences, 32(4). en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16027
dc.description.abstract Herbal remedies like the Thymus serpyllum L. is useful in traditional medicine for the treatment of many diseases especially congestion, and bronchitis. The purpose of this study was to formulate a micro-emulsion, a gel and an ointment containing the plant hydro distilled thymus oil extracted from Thymus serpyllum L. collected from Ziarat, Balochistan. The prepared formulations were subjected to in-vitro and ex vivo study release, High performance Liquid Chromatography (HPLC), Thin Layer Chromatography (TLC), to justify their suitability for topical use. The in-vitro and ex-Vivo release was studied using Franz Cells and using two different kinds of membrane synthetic dialysis cellulose membrane and natural rabbit skin, and the amount of drug released was determined by HPLC at λ 274nm. The three formulations result obtained through dialysis cellulose membrane showed the faster release than the natural rabbit skin. However, the micro-emulsion, gel formulation showed the same release except ointment. The release from the above mentioned formulation can be arranged in the following descending order. micro-emulsion > Gel > Ointment. The best fit of release kinetics was achieved by Krosmeyer- Peppas, the TLC and HPLC identifies the Thymol, isolation and quantification of the marker. This study demonstrates that it is necessary to assess the impact of release and permeability pattern of different formulations. In vitro and ex-vivo diffusion cell experiments can be utilized to develop formulations of traditional medicines identifies. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Thymus serpyllum L en_US
dc.subject Thymus oil en_US
dc.subject topical preparations en_US
dc.subject dialysis cellulose membrane en_US
dc.title Extraction, formulation and characterization of an in vitro and ex-vivo evaluation of Thymus serpyllum L. (Thymus oil) from topical preparations using dialysis cellulose membrane and natural rabbit skin en_US
dc.type Article en_US


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