dc.contributor.author |
Aisha Qayyum |
|
dc.contributor.author |
Muzammil Hasan Najmi |
|
dc.contributor.author |
Syed Irfan Raza |
|
dc.contributor.author |
Rajput, Tausif Ahmad |
|
dc.contributor.author |
Abdul Khaliq Naveed |
|
dc.date.accessioned |
2023-01-16T09:26:25Z |
|
dc.date.available |
2023-01-16T09:26:25Z |
|
dc.date.issued |
2019-07-16 |
|
dc.identifier.citation |
Qayyum, A., Najmi, M. H., Raza, S. I., Rajput, T. A., & Naveed, A. K. (2019). Relationship of S/R warfarin ratio with CYP2C9 genotypes in Pakistani population. Pakistan Journal of Pharmaceutical Sciences, 32(4). |
en_US |
dc.identifier.issn |
1011-601X |
|
dc.identifier.uri |
http://142.54.178.187:9060/xmlui/handle/123456789/16031 |
|
dc.description.abstract |
Warfarin is administered as a racemic preparation of R- and S-enantiomers. S-warfarin is more potent than Rwarfarin, so changes in blood levels of S-warfarin affect the anticoagulant response. This study was carried out to determine the effect of CYP2C9*2 and CYP2C9*3 polymorphisms on S/R warfarin ratio. A single blood sample was collected 12-16 hours after drug administration from 170 stable patients fulfilling the criteria. Genotyping of the CYP2C9 polymorphisms was done by polymerase chain reaction-restriction fragment length polymorphism assay. Sand R-warfarin enantiomers extraction from plasma was accomplished by a validated HPLC method. The concentration of S-warfarin was significantly different among CYP2C9 genotypes (p =0.018) whereas there was no effect on Rwarfarin (p =0.134). There was statistically significant effect of different CYP2C9 genotypes on S/R warfarin ratio (p=0.000). It is concluded that CYP2C9 polymorphisms influence CYP2C9 enzymatic activity in turn affecting Swarfarin levels but not R-warfarin, thus leading to different S/R warfarin enantiomers ratio among different CYP2C9 genotypes. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi |
en_US |
dc.subject |
S-Warfarin Enantiomer |
en_US |
dc.subject |
R-Warfarin Enantiomer |
en_US |
dc.subject |
CYP2C9 Genotypes |
en_US |
dc.subject |
HPLC |
en_US |
dc.subject |
PCR-RFLP |
en_US |
dc.title |
Relationship of S/R warfarin ratio with CYP2C9 genotypes in Pakistani population |
en_US |
dc.type |
Article |
en_US |