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Relationship of S/R warfarin ratio with CYP2C9 genotypes in Pakistani population

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dc.contributor.author Aisha Qayyum
dc.contributor.author Muzammil Hasan Najmi
dc.contributor.author Syed Irfan Raza
dc.contributor.author Rajput, Tausif Ahmad
dc.contributor.author Abdul Khaliq Naveed
dc.date.accessioned 2023-01-16T09:26:25Z
dc.date.available 2023-01-16T09:26:25Z
dc.date.issued 2019-07-16
dc.identifier.citation Qayyum, A., Najmi, M. H., Raza, S. I., Rajput, T. A., & Naveed, A. K. (2019). Relationship of S/R warfarin ratio with CYP2C9 genotypes in Pakistani population. Pakistan Journal of Pharmaceutical Sciences, 32(4). en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16031
dc.description.abstract Warfarin is administered as a racemic preparation of R- and S-enantiomers. S-warfarin is more potent than Rwarfarin, so changes in blood levels of S-warfarin affect the anticoagulant response. This study was carried out to determine the effect of CYP2C9*2 and CYP2C9*3 polymorphisms on S/R warfarin ratio. A single blood sample was collected 12-16 hours after drug administration from 170 stable patients fulfilling the criteria. Genotyping of the CYP2C9 polymorphisms was done by polymerase chain reaction-restriction fragment length polymorphism assay. Sand R-warfarin enantiomers extraction from plasma was accomplished by a validated HPLC method. The concentration of S-warfarin was significantly different among CYP2C9 genotypes (p =0.018) whereas there was no effect on Rwarfarin (p =0.134). There was statistically significant effect of different CYP2C9 genotypes on S/R warfarin ratio (p=0.000). It is concluded that CYP2C9 polymorphisms influence CYP2C9 enzymatic activity in turn affecting Swarfarin levels but not R-warfarin, thus leading to different S/R warfarin enantiomers ratio among different CYP2C9 genotypes. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject S-Warfarin Enantiomer en_US
dc.subject R-Warfarin Enantiomer en_US
dc.subject CYP2C9 Genotypes en_US
dc.subject HPLC en_US
dc.subject PCR-RFLP en_US
dc.title Relationship of S/R warfarin ratio with CYP2C9 genotypes in Pakistani population en_US
dc.type Article en_US


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