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REPORT Biochemical and molecular characterization of catalase enzyme in the saprobic fungus: Sordaria fimicola

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dc.contributor.author Muhammad Ishfaq
dc.contributor.author Nasir Mahmood
dc.contributor.author Muhammad Akbar
dc.contributor.author Idrees Ahmad Nasir
dc.contributor.author Muhammad Saleem
dc.date.accessioned 2023-01-19T04:04:28Z
dc.date.available 2023-01-19T04:04:28Z
dc.date.issued 2019-08-04
dc.identifier.citation Ishfaq, M., Mahmood, N., Akbar, M., Nasir, I. A., & Saleem, M. (2019). Biochemical and molecular characterization of catalase enzyme in the saprobic fungus: Sordaria fimicola. Pakistan Journal of Pharmaceutical Sciences, 32(4). en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16060
dc.description.abstract Fungi have been used in modern scientific research due to their high potential for different enzymes production based on genomic features. The great proportion of soil mycoflora represented by saprobic fungi plays an important role in decomposition, thus contribute to the global carbon cycle. Sordaria fimicola strains (n= 61) collected from different environments were evaluated for catalase enzyme activity at first stage. Among all 61 isolates of S. fimicola, five strains viz. S1, S2, N7, N6 and SF13 were found to be most efficient in catalase enzyme activity. The complete catalase gene including exons and introns was amplified and sequenced from the most efficient strains of S. fimicola and then submitted in the NCBI data base under accession numbers KM282183, KM282184, KM282186, KM282185 and KM282182 for strains S1, S2, N7, N6 and SF13 respectively. The significant differences in the genes sequences and theoretically translated proteins were observed for all five strains of S. fimicola. As regards catalase enzyme activity, S. fimicola strains were found comparable to the Aspergillus niger strains, therefore being a saprophytic fungus with short life cycle S. fimicola can become a fungus of choice to produce catalase enzyme at large scale. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Sordaria fimicola en_US
dc.subject catalase assay en_US
dc.subject ribotyping en_US
dc.subject gene sequencing en_US
dc.title REPORT Biochemical and molecular characterization of catalase enzyme in the saprobic fungus: Sordaria fimicola en_US
dc.type Article en_US


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