dc.contributor.author |
Zeb-un-nisa |
|
dc.contributor.author |
Muhammad Harris Shoaib |
|
dc.contributor.author |
Syed Imran Ali |
|
dc.contributor.author |
Mehwish Rizvi |
|
dc.contributor.author |
Huma Ali |
|
dc.contributor.author |
Rasheeda Fatima |
|
dc.contributor.author |
Khan, Maqsood Ahmed |
|
dc.contributor.author |
Sadia Suri Kashif |
|
dc.date.accessioned |
2023-01-19T04:07:45Z |
|
dc.date.available |
2023-01-19T04:07:45Z |
|
dc.date.issued |
2018-05-02 |
|
dc.identifier.citation |
Shoaib, M. H., Ali, S. I., Rizvi, M., Ali, H., Fatima, R., Khan, M. A., & Kashif, S. S. (2018). Development and validation of RP-HPLC method with UV detection to determine and quantify dimenhydrinate in human plasma. Pakistan Journal of Pharmaceutical Sciences, 31. |
en_US |
dc.identifier.issn |
1011-601X |
|
dc.identifier.uri |
http://142.54.178.187:9060/xmlui/handle/123456789/16063 |
|
dc.description.abstract |
A simple, sensitive and rigorous method for estimation of dimenhydrinate in human plasma was searched and
its validation was carried out. LLE (Liquid-Liquid extraction) of analyte with mixture of Hexane and ethyl acetate (1:1 v/v) was carried out for the preparation of Plasma Samples, Chromatographic elution of dimenhydrinate was conducted in human plasma and mobile phase with C-18 bonda Pack column (10µm; 250 × 4.6), using a mobile phase consisting a solution of ammonium bicarbonate in water and methanol at a flow rate of 0.5ml/minute with UV detection at 229 nm. The resolution of dimenhydrinate was well performed from plasma components. This method was validated and exhibited linearity with concentration range of 6 to 380ng/ml of dimenhydrinate in plasma. The Intra day precision was 89.2 to 96.89% and Inter day precision was 88.6% to 93.26%, the average recovery of dimenhydrinate was 97.02%. The efficacy of extraction was proved by above mentioned results. 2ng/ml and 6ng/ml, were appraised as the LOD and LOQ of dimenhydrinate, stability studies disclosed that dimenhydrinate exhibited stability in Plasma after Freeze & thaw cycles and upon -20o C storage, the method was developed well. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi |
en_US |
dc.subject |
Dimenhydrinate |
en_US |
dc.subject |
human plasma |
en_US |
dc.subject |
HPLC |
en_US |
dc.subject |
limit of detection |
en_US |
dc.subject |
analyte |
en_US |
dc.title |
Development and validation of RP- HPLC method with UV detection to determine and quantify dimenhydrinate in human plasma |
en_US |
dc.type |
Article |
en_US |