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Development and validation of RP- HPLC method with UV detection to determine and quantify dimenhydrinate in human plasma

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dc.contributor.author Zeb-un-nisa
dc.contributor.author Muhammad Harris Shoaib
dc.contributor.author Syed Imran Ali
dc.contributor.author Mehwish Rizvi
dc.contributor.author Huma Ali
dc.contributor.author Rasheeda Fatima
dc.contributor.author Khan, Maqsood Ahmed
dc.contributor.author Sadia Suri Kashif
dc.date.accessioned 2023-01-19T04:07:45Z
dc.date.available 2023-01-19T04:07:45Z
dc.date.issued 2018-05-02
dc.identifier.citation Shoaib, M. H., Ali, S. I., Rizvi, M., Ali, H., Fatima, R., Khan, M. A., & Kashif, S. S. (2018). Development and validation of RP-HPLC method with UV detection to determine and quantify dimenhydrinate in human plasma. Pakistan Journal of Pharmaceutical Sciences, 31. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16063
dc.description.abstract A simple, sensitive and rigorous method for estimation of dimenhydrinate in human plasma was searched and its validation was carried out. LLE (Liquid-Liquid extraction) of analyte with mixture of Hexane and ethyl acetate (1:1 v/v) was carried out for the preparation of Plasma Samples, Chromatographic elution of dimenhydrinate was conducted in human plasma and mobile phase with C-18 bonda Pack column (10µm; 250 × 4.6), using a mobile phase consisting a solution of ammonium bicarbonate in water and methanol at a flow rate of 0.5ml/minute with UV detection at 229 nm. The resolution of dimenhydrinate was well performed from plasma components. This method was validated and exhibited linearity with concentration range of 6 to 380ng/ml of dimenhydrinate in plasma. The Intra day precision was 89.2 to 96.89% and Inter day precision was 88.6% to 93.26%, the average recovery of dimenhydrinate was 97.02%. The efficacy of extraction was proved by above mentioned results. 2ng/ml and 6ng/ml, were appraised as the LOD and LOQ of dimenhydrinate, stability studies disclosed that dimenhydrinate exhibited stability in Plasma after Freeze & thaw cycles and upon -20o C storage, the method was developed well. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Dimenhydrinate en_US
dc.subject human plasma en_US
dc.subject HPLC en_US
dc.subject limit of detection en_US
dc.subject analyte en_US
dc.title Development and validation of RP- HPLC method with UV detection to determine and quantify dimenhydrinate in human plasma en_US
dc.type Article en_US


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