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Study of biological activity and targeted distribution of catesbeianalectin in mouse tissue

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dc.contributor.author Zhao, Rui-Li
dc.contributor.author Lin Zhu
dc.contributor.author Liu, Meng-Yue
dc.contributor.author Jin, Tian-Ming
dc.contributor.author Ma, Ji-Fei
dc.contributor.author Hu, Ye
dc.contributor.author Wang, Xuan
dc.contributor.author Qin, Shun-Yi
dc.contributor.author Chen, Jian-Bao
dc.date.accessioned 2023-01-19T04:28:16Z
dc.date.available 2023-01-19T04:28:16Z
dc.date.issued 2018-05-07
dc.identifier.citation Zhao, R. L., Zhu, L., Liu, M. Y., Jin, T. M., Ma, J. F., Hu, Y., ... & Chen, J. B. (2018). Study of biological activity and targeted distribution of catesbeianalectin in mouse tissue. Pakistan Journal of Pharmaceutical Sciences, 31. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16068
dc.description.abstract Lectin has attracted attention because of its ability to serve as a carrier for targeted drug delivery. Large lectins isolated from marine invertebrates and crustaceans have strong immunogenicity and adverse effects, which limit their usefulness. This study reports the identification of catesbeianalectin via screening a bullfrog skin cDNA library. The catesbeianalectin polypeptide has a molecular weight of 1.47 kD, making it the smallest known lectin in terms of molecular weight. Circular dichroism analysis showed a PPII helix secondary structure. Catesbeianalectin strongly induces agglutination of rabbit erythrocytes and a variety of pathogens include Staphylococcus aureus, Streptococcus suis type 2, Actinobacillus pleuropneumoniae, and piglet paratyphoid Salmonella. The mean serum titer in catesbeianalectin-immunized Balb/c mice was 1:25, which was significantly lower than that of positive controls immunized with wheat germ agglutinin. Surface plasmon resonance indicated an S-type lectin. 125I-labeled catesbeianalectin did not pass the blood-brain barrier. This study provides a basis for further research on the potential of catesbeianalectin as a carrier in targeted drug delivery. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Catesbeianalectin en_US
dc.subject circular dichroism en_US
dc.subject immunogenicity en_US
dc.subject surface plasmon resonance imaging en_US
dc.subject 125I-labeled en_US
dc.title Study of biological activity and targeted distribution of catesbeianalectin in mouse tissue en_US
dc.type Article en_US


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