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Quantitative determination of deferiprone in human plasma by reverse phase high performance liquid chromatography and its application to pharmacokinetic study

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dc.contributor.author Mateen Abbas
dc.contributor.author Rakhshanda Nawaz
dc.contributor.author Tahira Iqbal
dc.contributor.author Muhammad Alim
dc.contributor.author Muhammad Rafique Asi
dc.date.accessioned 2023-01-20T05:05:13Z
dc.date.available 2023-01-20T05:05:13Z
dc.date.issued 2012-04-08
dc.identifier.citation Abbas, M., Nawaz, R., Iqbal, T., Alim, M., & Asi, M. R. (2012). Quantitative determination of deferiprone in human plasma by reverse phase high performance liquid chromatography and its application to pharmacokinetic study. Pakistan journal of pharmaceutical sciences, 25(2). en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16170
dc.description.abstract Deferiprone (1, 2 dimethyl-3-hydroxypyrid-4-one) is considered to be the standard iron chelator. Pharmacokinetic studies of generic formulations are required in local condition before placed on the market. High performance liquid chromatographic (HPLC) method was used for quantification of deferiprone in human plasma using UV/VIS detector. Chromatographic separation was carried out on C18 column, with a mobile phase of methanol-buffer (18:82, v/v), pH 3.5, and caffeine was used as an internal standard. The calibration curve was linear over the range 0.25- 10 µg/mL in human plasma (R2 = 0.9994). After oral administration of deferiprone (500 mg) to human, the plasma concentration-time curve of deferiprone was conformed to two-compartment open model. The deferiprone plasma concentration showed a rapid absorption and average area under the plasma concentration-time curve (AUC) of deferiprone was 17.0 ± 1.23 h.µg/mL. Average absorption and elimination half-life values of deferiprone of 24 volunteers were 0.62 ± 0.12 and 2.65 ± 0.43 hours. This study confirms the rapid absorption of deferiprone in humans. AUC was similar to that previously reported but Cmax was slightly lower than that stated in the literature. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Deferiprone en_US
dc.subject HPLC en_US
dc.subject human en_US
dc.subject validation en_US
dc.subject pharmacokinetics en_US
dc.title Quantitative determination of deferiprone in human plasma by reverse phase high performance liquid chromatography and its application to pharmacokinetic study en_US
dc.type Article en_US


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