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Simultaneous determination of triacetin, acetic ether, butyl acetate and amorolfine hydrochloride in amorolfine liniment by HPLC

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dc.contributor.author Gao, Yuan
dc.contributor.author LI, Li
dc.contributor.author Zhang, Jianjun
dc.contributor.author Shu, Wenjuan
dc.contributor.author Gao, Liqiong
dc.date.accessioned 2023-01-20T05:15:25Z
dc.date.available 2023-01-20T05:15:25Z
dc.date.issued 2012-04-16
dc.identifier.citation Gao, Y., Li, L. I., Zhang, J., Shu, W., & Gao, L. (2012). Simultaneous determination of triacetin, acetic ether, butyl acetate and amorolfine hydrochloride in amorolfine liniment by HPLC. Pakistan Journal of Pharmaceutical Sciences, 25(2). en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16181
dc.description.abstract A simple, rapid, specific and precise reversed-phase high-performance liquid chromatographic method was developed for simultaneous estimation of triacetin, acetic ether, butyl acetate and amorolfine in marketed pharmaceutical liniment. Chromatographic separation was performed on a Shimadzu VP-ODS C18 column using the mixture of citric acid-hydrochloric acid-sodium hydrate buffer (pH 3.0), acetonitrile and methanol (32:30:38) as the mobile phase at a flow rate of 1.0 mL/min with UV-detection at 215 nm. The method separated the four components simultaneously in less than 10 min. The validation of the method was performed with respect to specificity, linearity, accuracy, and precision. The calibration curves were linear in the range of 35.1-81.9 µg/mL for triacetin, 431.1-1005.9 µg/mL for acetic ether, 167.0-389.7 µg/mL for butyl acetate and 151.0-352.3 µg/mL for amorolfine. The mean 100% spiked recovery for triacetin, acetic ether, butyl acetate and amorolfine is 99.43 ± 0.42, 101.5±1.09, 101.4±1.02 and 100.8±0.69, respectively. The intra-day and inter-day relative standard deviation values were <2.0%. The limits of detection of these compounds ranged from 0.08 to 5.88 ng. The utility of the procedure was verified by its application to the commercial liniment. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Amorolfine en_US
dc.subject liniment en_US
dc.subject HPLC en_US
dc.subject triacetin en_US
dc.subject acetic ether en_US
dc.subject butyl acetate en_US
dc.title Simultaneous determination of triacetin, acetic ether, butyl acetate and amorolfine hydrochloride in amorolfine liniment by HPLC en_US
dc.type Article en_US


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