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Role of prostaglandin H synthase in activation of acrylonitrile to cyanide

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dc.contributor.author Abbasi-Al, Ahmed Fahad
dc.contributor.author Esmat Ahmed
dc.contributor.author Mohamadin, Mohamed Ahmed
dc.contributor.author Abdel Naim, Ashraf Bahyeldeen
dc.date.accessioned 2023-01-20T05:45:43Z
dc.date.available 2023-01-20T05:45:43Z
dc.date.issued 2018-07-20
dc.identifier.citation Al-Abbasi, F. A., Esmat, A., Mohamadin, A. M., & Abdel-Naim, A. B. (2018). Role of prostaglandin H synthase in activation of acrylonitrile to cyanide. Pakistan Journal of Pharmaceutical Sciences, 31(4). en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16200
dc.description.abstract The present study aimed at investigating the in-vitro oxidation of acrylonitrile (ACN) to cyanide (CN−) by prostaglandin H synthase (PHS). Detection of CN− is considered a marker for free radical intermediates involved in ACN-induced toxicity. First, most favorable circumstances for ACN oxidation were characterized: pH (4.5), temperature (37ºC) and time of incubation (60 min.). In addition, the concentrations of ACN, PHS and H2O2 in incubation mixtures were assessed for further reaction characterization. The reaction maximum velocity (Vmax) was calculated to be 582.75 pmol CN−/mL/min and the Michaelis-Menten constant (Km) was 149.25 µmol ACN. Adding PHS inhibitors; resveratrol, quercetin, indomethacin or troloc-C to the reaction mixtures significantly reduced the rate of ACN oxidation. In conclusion, the present study demonstrates the ability of PHS to oxidize ACN to CN− and provides a clue for the explanation of ACN target toxicity. en_US
dc.language.iso en en_US
dc.publisher Karachi: Pakistan Botanical Society, University of Karachi en_US
dc.subject Acrylonitrile en_US
dc.subject prostaglandin H synthase en_US
dc.subject cyanide. en_US
dc.title Role of prostaglandin H synthase in activation of acrylonitrile to cyanide en_US
dc.type Article en_US


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