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Research on chemotherapy efficacy of twist gene on cervical cancer cells to paclitaxel

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dc.contributor.author Sun, Zhenchang
dc.contributor.author Zhang, Dan
dc.contributor.author Cui, Yingying
dc.contributor.author Cheng, Liangxing
dc.contributor.author Cao, Jingyu
dc.contributor.author Wu, Xiaolong
dc.date.accessioned 2023-01-20T05:57:20Z
dc.date.available 2023-01-20T05:57:20Z
dc.date.issued 2014-09-22
dc.identifier.citation Sun, Z., Zhang, D., Cui, Y., Cheng, L., Cao, J., & Wu, X. (2014). Research on chemotherapy efficacy of twist gene on cervical cancer cells to paclitaxel. Pakistan Journal of Pharmaceutical Sciences, 27(5). en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16228
dc.description.abstract The silent Twist gene may increase the sensitivity of cervical cancer cells chemotherapy to paclitaxel, thus was a new idea to improve the efficacy of cancer chemotherapy. The aim was to explore chemotherapy sensitivity of silent Twist gene increased cervical cancer cells to paclitaxel through study the proliferation and apoptosis of cervical cancer Twist gene after paclitaxel treatment. Cervical carcinoma Caski cells and Hela cells was cultured in vitro, mRNA gene expression was detected by using semi-quantitative, fluorescence quantitative PCR, and transferred to Caski cells transiently, and affected with paclitaxel solution of five kinds of different concentrations of 0.001, 0.01, 0.1, 1, 10 umol/L respectively. Then the results of <0.05). Every 12h after 36h, the expression inhibition rate in two groups of Caski cells that has transfected this study was Twist gene expression in Caski cells was higher than in Hela cells, which was of significant difference (p siRNA1 and siRNA2 were 20.3%, 38.2%, 33%, 24%, 68.6%, 50.8% respectively. After 48h in five different concentrations of paclitaxel effect, the cell growth inhibition rate of group siRNA2 with the best transfection efficiency was obviously higher than that of negative control group and blank control group, and the growth inhibition rates showed concentration dependence (p<0.05). It can be concluded that Twist gene in Caski cell was of high expression and the silent Twist gene could inhibit Caski cell proliferation and promote its apoptosis, thus to improve the chemotherapy sensitivity of Caski cells. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Paclitaxel en_US
dc.subject cell growth inhibition rates en_US
dc.subject Caski cells en_US
dc.subject silent Twist gene en_US
dc.title Research on chemotherapy efficacy of twist gene on cervical cancer cells to paclitaxel en_US
dc.type Article en_US


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