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The surface protease OmpT serves as Escherichia coli K1 adhesion in binding to human brain micro vascular endothelial cells

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dc.contributor.author Wan, Lei
dc.contributor.author Guo, Yan
dc.contributor.author Hui, Chang-Ye
dc.contributor.author Liu, Xiao-Lu
dc.contributor.author Zhang, Wen-Bing
dc.contributor.author Cao, Hong
dc.date.accessioned 2023-01-20T05:58:18Z
dc.date.available 2023-01-20T05:58:18Z
dc.date.issued 2014-05-01
dc.identifier.citation Wan, L., Guo, Y., Hui, C. Y., Liu, X. L., Zhang, W. B., & Cao, H. (2014). The surface protease ompT serves as Escherichia coli K1 adhesin in binding to human brain micro vascular endothelial cells. Pak. J. Pharm. Sci, 27, 617-624. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16231
dc.description.abstract Escherichia coli (E. coli) K1 is the most common bacteria that cause meningitis in the neonatal period. But it’s not entirely clear about how E. coli crosses the blood-brain barrier. The features of the OmpT deletion in meningitic E. coli infection were texted in vitro. In comparison with the parent strain, the isogenic OmpT deletion mutant was significantly less adhesive to human brain microvascular endothelial cells (HBMEC). The adhesion-deficient phenotype of the mutant was restored to the level of the wild-type by complementing with low-level OmpT expression plasmid. Interestingly, the adhesion was enhanced by point mutation at the OmpT proposed catalytic residue D85. Compared with the poor adhesive activity of bovine serum albumin-coated fluorescent beads, recombinant OmpT or catalytically inactive variant of OmpT-coated beads bound to HBMEC monolayer effectively. Our study suggests that OmpT is important for bacterial adhesion while entering into central nervous system, and the adhesion does not involve in the proteolytic activity of OmpT. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject OmpT en_US
dc.subject adhesion en_US
dc.subject meningitis en_US
dc.subject brain microvascular endothelial cells en_US
dc.subject E. coli K1 en_US
dc.title The surface protease OmpT serves as Escherichia coli K1 adhesion in binding to human brain micro vascular endothelial cells en_US
dc.type Article en_US


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