dc.contributor.author |
Jia, Dongfang |
|
dc.contributor.author |
Zheng, Chenna |
|
dc.contributor.author |
Feng, Jingxian |
|
dc.contributor.author |
Zou, Jin |
|
dc.contributor.author |
Diao, Yong |
|
dc.date.accessioned |
2023-01-20T05:58:57Z |
|
dc.date.available |
2023-01-20T05:58:57Z |
|
dc.date.issued |
2014-05-03 |
|
dc.identifier.citation |
Jia, D., Zheng, C., Feng, J., Zou, J., & Diao, Y. (2014). Plasmid mediated kallistain gene expression via intramuscular electroporation delivery in vivo for treatment of NCI-H446 subcutaneous xenograft tumor. Pakistan Journal of Pharmaceutical Sciences, 27. |
en_US |
dc.identifier.issn |
1011-601X |
|
dc.identifier.uri |
http://142.54.178.187:9060/xmlui/handle/123456789/16233 |
|
dc.description.abstract |
Kallistatin (KAL) is a novel anti-tumor protein with anti-angiogenic activity. The aim of this study was to
investigate whether intramuscular injection of KAL plasmid DNA by electroporation could inhibit NCI-H446
subcutaneous xenograft tumor growth in mice. The tumor model of BALB/c nude mice was induced by subcutaneous inoculation of 5×106 NCI-H446 cells into the mice right flank. The next day, naked plasmid pEGFP or pKAL was electrotransferred into the skeletal muscle of nude mice (n=6 for each group), with the optimized electroporation conditions. Tumor cells migration were assessed by E-cadherin staining; proliferation was determined by anti-Ki-67 staining; and apoptosis was assayed via TUNEL, tumor microvessel density (MVD) was examined by anti-CD34 staining to evaluate the angiogenesis of tumor. Compared to the pEGFP treating group, tumor growth was inhibited by 85% (pEGFP group: 486±187 mm3 , pKAL group: 71±33 mm3 ) at day 42, the MVD of tumor tissues was significantly decreased, and tumor cellular proliferation was also inhibited. The results indicate that this therapeutic strategy might serve as a promising approach for cancer clinical therapy. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi |
en_US |
dc.subject |
Kallistain |
en_US |
dc.subject |
xenograft tumor |
en_US |
dc.subject |
electroporation |
en_US |
dc.subject |
gene therapy |
en_US |
dc.title |
Plasmid mediated kallistain gene expression via intramuscular electroporation delivery in vivo for treatment of NCI-H446 subcutaneous xenograft tumor |
en_US |
dc.type |
Article |
en_US |