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Plasmid mediated kallistain gene expression via intramuscular electroporation delivery in vivo for treatment of NCI-H446 subcutaneous xenograft tumor

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dc.contributor.author Jia, Dongfang
dc.contributor.author Zheng, Chenna
dc.contributor.author Feng, Jingxian
dc.contributor.author Zou, Jin
dc.contributor.author Diao, Yong
dc.date.accessioned 2023-01-20T05:58:57Z
dc.date.available 2023-01-20T05:58:57Z
dc.date.issued 2014-05-03
dc.identifier.citation Jia, D., Zheng, C., Feng, J., Zou, J., & Diao, Y. (2014). Plasmid mediated kallistain gene expression via intramuscular electroporation delivery in vivo for treatment of NCI-H446 subcutaneous xenograft tumor. Pakistan Journal of Pharmaceutical Sciences, 27. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16233
dc.description.abstract Kallistatin (KAL) is a novel anti-tumor protein with anti-angiogenic activity. The aim of this study was to investigate whether intramuscular injection of KAL plasmid DNA by electroporation could inhibit NCI-H446 subcutaneous xenograft tumor growth in mice. The tumor model of BALB/c nude mice was induced by subcutaneous inoculation of 5×106 NCI-H446 cells into the mice right flank. The next day, naked plasmid pEGFP or pKAL was electrotransferred into the skeletal muscle of nude mice (n=6 for each group), with the optimized electroporation conditions. Tumor cells migration were assessed by E-cadherin staining; proliferation was determined by anti-Ki-67 staining; and apoptosis was assayed via TUNEL, tumor microvessel density (MVD) was examined by anti-CD34 staining to evaluate the angiogenesis of tumor. Compared to the pEGFP treating group, tumor growth was inhibited by 85% (pEGFP group: 486±187 mm3 , pKAL group: 71±33 mm3 ) at day 42, the MVD of tumor tissues was significantly decreased, and tumor cellular proliferation was also inhibited. The results indicate that this therapeutic strategy might serve as a promising approach for cancer clinical therapy. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Kallistain en_US
dc.subject xenograft tumor en_US
dc.subject electroporation en_US
dc.subject gene therapy en_US
dc.title Plasmid mediated kallistain gene expression via intramuscular electroporation delivery in vivo for treatment of NCI-H446 subcutaneous xenograft tumor en_US
dc.type Article en_US


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