PASTIC Dspace Repository

High-level expression of fusion peptide Dybowskin-2CDYa and hEGF in E. coli and its dual function

Show simple item record

dc.contributor.author Jin, LiLi
dc.contributor.author Jiang, Chao
dc.contributor.author Zhang, XueMin
dc.contributor.author Wang, Zheng
dc.contributor.author Hou, Xiao
dc.contributor.author Wang, QiuYu
dc.date.accessioned 2023-01-20T05:59:17Z
dc.date.available 2023-01-20T05:59:17Z
dc.date.issued 2014-05-04
dc.identifier.citation Jin, L., Jiang, C., Zhang, X., Wang, Z., Hou, X., & Wang, Q. (2014). High-level expression of fusion peptide Dybowskin-2CDYa and hEGF in E. coli and its dual function. Pakistan Journal of Pharmaceutical Sciences, 27. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16234
dc.description.abstract Dybowskin-2CDYa (Dy2), with a broad antimicrobial spectrum and low hemolytic feature, is a newly discovered type of antimicrobial peptides from Rana dybowskii. In order to get a dual function peptide which inhibits bacterial growth and promotes cell proliferation, we cloned the gene of Dy2and hEGF (human epidermal growth factor) into the prokaryotic expression vector pET-30a(+). With isopropyl-β-D-thiogalactoside (IPTG) induction, a 13.7KDa peptide with a 6×His tag was highly expressed in the form of inclusion in E. coli BL2l (DE3). SDS-PAGE and westernblot confirmed the expression of the fusion peptide hEGF-Dy2. Under the optimized condition of 1.0mmol/L IPTG induction and incubation time 4h at 37o, the yield of hEGF- y2reached 30mg/L following purification on nickelnitrilotriacetic acid (Ni-NTA) metal affinity chromatography matrices. The purified fusion peptide showed significant antibacterial activities against Staphylococcus aureus, Escherichia coli O157, Pseudomonas aeruginosa and proliferating activities on NIH3T3. These results indicated that the fusion peptide might have a good prospect in therapy of trauma and burns. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Antimicrobial peptide en_US
dc.subject Dybowskin-2CDYa en_US
dc.subject human epidermal growth factor en_US
dc.subject fusion expression en_US
dc.subject antibacterial activities en_US
dc.subject proliferating activities en_US
dc.title High-level expression of fusion peptide Dybowskin-2CDYa and hEGF in E. coli and its dual function en_US
dc.type Article en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account