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Mutagenic breeding of Quambalaria cyanescens strain for the production of milk-clotting enzymes

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dc.contributor.author Wang, Chengzhong
dc.contributor.author Rao, Hongyan
dc.contributor.author Zhou, Zhongkai
dc.contributor.author Zhang, Yan
dc.date.accessioned 2023-01-20T06:00:26Z
dc.date.available 2023-01-20T06:00:26Z
dc.date.issued 2014-05-06
dc.identifier.citation Wang, C., Rao, H., Zhou, Z., & Zhang, Y. (2014). Mutagenic breeding of Quambalaria cyanescens strain for the production of milk-clotting enzymes. Pakistan Journal of Pharmaceutical Sciences, 27. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16236
dc.description.abstract A strain for producing rennet was screened from red kojic rice and identified as Quambalaria cyanescens. In the current work, various mutagenic treatments, such as UV, diethyl sulfate and their combinations were applied for improving the production of milk-clotting enzymes. The mutagenic conditions were optimized based on the fatality rate of the strain. A significant increasing in the productivity of the fungal rennet produced from the strain Quambalaria cyanescens was achieved and its milk-clotting activity (MCA) was increased from 57 to 117 (SU mL-1) through the mutagenic breeding. Further study showed that MCA was greatly inhibited (P<0.0001) by pepstain A, indicating it belongs to an aspartic acid protease, but absence of serine protease, metalloproteinase and cysteine protease. The mutated strain with the highest activity of milk-clotting enzymes showed a stable capacity to produce rennet through the test of its heritability. This is the first report of the breeding study of Quambalaria cyanescens for its capacity to produce microbial rennet. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Quambalaria cyanescens en_US
dc.subject rennet en_US
dc.subject mutagenic breeding en_US
dc.subject milk-clotting activity en_US
dc.title Mutagenic breeding of Quambalaria cyanescens strain for the production of milk-clotting enzymes en_US
dc.type Article en_US


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